2. 重组蛋白
  3. Cytokines and Growth Factors
  4. Ephrin/Eph Family
  5. Ephrins
  6. Ephrin-A3
  7. Ephrin-A3/EFNA3 蛋白, Mouse (HEK293, Fc)

Ephrin-A3/EFNA3 蛋白, Mouse (HEK293, Fc)

目录号: HY-P70195
产品使用指南

Ephrin-A3/EFNA3 蛋白是一种 GPI 结合配体，与 Eph 受体相互作用，在神经元、血管和上皮发育中发挥关键作用。它结合相邻的 Eph 受体，启动双向信号传导。Ephrin-A3/EFNA3 还激活 EPHA8，有助于其迁移、排斥和粘附的调节功能。Ephrin-A3/EFNA3 蛋白, Mouse (HEK293, Fc) 是重组的 Ephrin-A3/EFNA3 蛋白，由 HEK293 表达，带有 C-hFc 标签。Ephrin-A3/EFNA3 蛋白, Mouse (HEK293, Fc) 全长 184 个氨基酸，分子量为 61-70 kDa。

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描述

Ephrin-A3/EFNA3 Protein, a GPI-bound ligand, interacts with Eph receptors, playing a critical role in neuronal, vascular, and epithelial development. It binds adjacent Eph receptors, initiating bidirectional signaling. Ephrin-A3/EFNA3 also activates EPHA8, contributing to its regulatory functions in migration, repulsion, and adhesion. Ephrin-A3/EFNA3 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived Ephrin-A3/EFNA3 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of Ephrin-A3/EFNA3 Protein, Mouse (HEK293, Fc) is 184 a.a., with molecular weight of 61-70 kDa.
研究背景

Ephrin-A3/EFNA3 蛋白是一种细胞表面 GPI 结合配体，通过与 Eph 受体（参与迁移、排斥和粘附的受体酪氨酸激酶家族）相互作用，在神经元、血管和上皮发育中发挥关键作用。它与相邻细胞上的 Eph 受体混杂结合，启动接触依赖性双向信号传导。受体的下游信号传导称为正向信号传导，而肝配蛋白配体的下游信号传导称为反向信号传导。Ephrin-A3/EFNA3 还与 EPHA8 相互作用并激活该受体，进一步促进其调节功能。
种属

Mouse
表达系统

HEK293
标签

C-hFc
蛋白编号

O08545 (Q23-G206)
基因 ID

13638  [NCBI]
蛋白结构
N-term
EFNA3 (Q23-G206)
Accession # O08545
hFc
C-term
同用名
rMuEphrin-A3, Fc; Ephrin-A3; EFL-2; EHK1 Ligand; EHK1-L; EPH-Related Receptor Tyrosine Kinase Ligand 3; LERK-3; EFNA3; EFL2; EPLG3; LERK3
氨基酸序列

QGPGGALGNRHAVYWNSSNQHLRREGYTVQVNVNDYLDIYCPHYNSSGPGGGAEQYVLYMVNLSGYRTCNASQGSKRWECNRQHASHSPIKFSEKFQRYSAFSLGYEFHAGQEYYYISTPTHNLHWKCLRMKVFVCCASTSHSGEKPVPTLPQFTMGPNVKINVLEDFEGENPQVPKLEKSISG

分子量

61-70 kDa
纯度

Greater than 95% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

Ephrin-A3/EFNA3 Protein, Mouse (HEK293, Fc) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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