1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Ephrin/Eph Family
  4. Ephrins
  5. Ephrin-B3
  6. Ephrin B3 蛋白, Rat (HEK293, His)

Ephrin B3 蛋白, Rat (HEK293, His)

目录号: HY-P73022
COA 产品使用指南

Ephrin B3 蛋白是肝配蛋白家族的成员,缺乏对特征注释传播至关重要的保守残基。这种独特的分子特征表明肝配蛋白家族内具有独特的功能特性和相互作用。研究保守残基中这种差异的具体作用和影响对于理解 Ephrin B3 的功能细微差别和生物过程中的细胞意义至关重要。Ephrin B3 蛋白, Rat (HEK293, His) 是重组的 肝配蛋白 B3 蛋白,由 HEK293 表达,带有 C-His 标签。Ephrin B3 蛋白, Rat (HEK293, His) 全长 197 个氨基酸,分子量约为 27-32 kDa。

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10 μg ¥700 In-stock
50 μg ¥1750 In-stock
100 μg ¥2800 In-stock
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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

Ephrin B3 Protein, a member of the ephrin family, lacks conserved residue(s) crucial for feature annotation propagation. This unique molecular profile suggests distinct functional properties and interactions within the ephrin family. Investigating the specific role and implications of this divergence in conserved residues is crucial to understand Ephrin B3's functional nuances and cellular significance in biological processes. Ephrin B3 Protein, Rat (HEK293, His) is the recombinant rat-derived Ephrin B3 protein, expressed by HEK293 , with C-His labeled tag. The total length of Ephrin B3 Protein, Rat (HEK293, His) is 197 a.a., with molecular weight of 27-32 kDa.

研究背景

Ephrin B3 蛋白是肝配蛋白家族的成员,其特征是不存在对特征注释的传播至关重要的保守残基。这一独特的特征表明肝配蛋白家族具有独特的分子特征,可能影响其功能特性和相互作用。这种保守残基差异的具体作用和影响值得进一步研究,以了解 Ephrin B3 在生物过程中的功能细微差别和细胞意义。

生物活性

Immobilized Rat Ephrin B3 at 10μg/mL (100μL/well) can bind biotinylated Mouse EphB3, the ED50 for this effect is 36.91 ng/mL.

种属

Rat

表达系统

HEK293

标签

C-6*His

蛋白编号

G3V7D4 (L28-S224)

基因 ID

360546  [NCBI]

蛋白结构
N-term
Ephrin B3 (L28-S224)
Accession # G3V7D4
His
C-term
同用名
Ephrin-B3; EPH-related receptor tyrosine kinase ligand 8; LERK-8; EFNB3; EPLG8
氨基酸序列

MGGPHFGPGGVQVGALLLLGFAGLVSGLSLEPVYWNSANKRFQAEGGYVLYPQIGDRLDLLCPRARPPGPHSSPSYEFYKLYLVGGAQGRRCEAPPAPNLLLTCDRPDLDLRFTIKFQEYSPNLWGHEFRSHHDYYIIATSDGTREGLESLQGGVCLTRGMKVLLRVGQSPRGGAVPRKPVSEMPMERDRGAAHSQEPGKDSIPGDPNSNATSRGAEGPLPPPS

分子量

Approximately 27-32 kDa due to the glycosylation.

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Ephrin B3 Protein, Rat (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Ephrin B3 Protein, Rat (HEK293, His)
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