1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. EPHX1 蛋白, Human (His-SUMO)

EPHX1 蛋白, Human (His-SUMO)

目录号: HY-P72184
COA 产品使用指南

EPHX1蛋白是一种重要的生物转化酶,通过催化水的转加来水解芳香烃和脂肪族环氧化物。这使得环氧化物的反应性降低并且水溶性更高。EPHX1 蛋白, Human (His-SUMO) 是重组的 EPHX1 蛋白,由 E. coli 表达,带有 N-6*His, N-SUMO 标签。EPHX1 蛋白, Human (His-SUMO) 全长 455 个氨基酸,分子量约为 ~66 kDa。

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规格 价格 是否有货 数量
20 μg ¥1250 In-stock
50 μg ¥2380 In-stock
100 μg ¥3800 询价
> 100 μg   询价  

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描述

The EPHX1 protein is an important biotransformation enzyme that hydrolyzes aromatic hydrocarbons and aliphatic epoxides by catalyzing the transaddition of water. This makes the epoxide less reactive and more water-soluble. EPHX1 Protein, Human (His-SUMO) is the recombinant human-derived EPHX1 protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag. The total length of EPHX1 Protein, Human (His-SUMO) is 455 a.a., with molecular weight of ~66 kDa.

研究背景

EPHX1 蛋白是一种重要的生物转化酶,促进芳烃和脂肪族环氧化物的水解。通过水的反式加成,EPHX1 催化这种转化,使环氧化物的反应性降低,水溶性提高。除了在异生物质代谢中的作用外,EPHX1 在内源性脂质的代谢途径中发挥着重要作用,特别是在含环氧化物的脂肪酸的加工中。值得注意的是,EPHX1 参与丰富的内源性大麻素 2-花生四烯酰甘油 (2-AG) 的代谢,将其转化为游离花生四烯酸 (AA) 和甘油。这些酶活性凸显了 EPHX1 在调节各种底物的生物利用度和反应性方面的多功能作用,有助于重要的细胞过程和脂质代谢。

生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

种属

Human

表达系统

E. coli

标签

N-6*His;N-SUMO

蛋白编号

P07099 (M1-Q455)

基因 ID
蛋白结构
N-term
6*His-SUMO
EPHX1 (M1-Q455)
Accession # P07099
C-term
同用名
EPHX 1; EPHX; Ephx1; EPOX; Epoxide hydratase; Epoxide hydrolase 1; Epoxide hydrolase 1 microsomal xenobiotic; Epoxide hydroxylase 1; Epoxide hydroxylase 1 microsomal xenobiotic; HYEP_HUMAN; HYL1; MEH; Microsomal epoxide hydrolase
氨基酸序列

MWLEILLTSVLGFAIYWFISRDKEETLPLEDGWWGPGTRSAAREDDSIRPFKVETSDEEIHDLHQRIDKFRFTPPLEDSCFHYGFNSNYLKKVISYWRNEFDWKKQVEILNRYPHFKTKIEGLDIHFIHVKPPQLPAGHTPKPLLMVHGWPGSFYEFYKIIPLLTDPKNHGLSDEHVFEVICPSIPGYGFSEASSKKGFNSVATARIFYKLMLRLGFQEFYIQGGDWGSLICTNMAQLVPSHVKGLHLNMALVLSNFSTLTLLLGQRFGRFLGLTERDVELLYPVKEKVFYSLMRESGYMHIQCTKPDTVGSALNDSPVGLAAYILEKFSTWTNTEFRYLEDGGLERKFSLDDLLTNVMLYWTTGTIISSQRFYKENLGQGWMTQKHERMKVYVPTGFSAFPFELLHTPEKWVRFKYPKLISYSYMVRGGHFAAFEEPELLAQDIRKFLSVLERQ

分子量

Approximately 66 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

无菌粉末

组分

Lyophilized from a 0.2 μm solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

EPHX1 Protein, Human (His-SUMO) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

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  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
EPHX1 Protein, Human (His-SUMO)
目录号:
HY-P72184
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