Erythropoietin/EPO 蛋白, Human (CHO)

Erythropoietin/EPO Protein, Human (CHO) protects the myocardium from ischemia-reperfusion injury and promotes beneficial remodeling.

促红细胞生成素 (EPO) 最初因其在促进红细胞存活和分化方面的关键激素作用而被鉴定，是庞大而多样的细胞因子超家族的成员。EPO 和 EPOR 是对组织缺氧的一般保护性反应的主要介质，其作用是通过使用涉及肾脏和骨髓的激素反馈控制系统调整循环红细胞量来维持足够的组织氧合。EPO 和 EPOR 也由其他组织和器官表达，包括大脑和心脏。EPO 还被证明可以刺激星形胶质细胞、内皮细胞、成肌细胞和体外^[1]保存的心肌细胞中的有丝分裂和信号传导。

The ED₅₀ is ≤2.299 ng/mL as measured in a cell proliferation assay using TF-1 human erythroleukemic cells, corresponding to a specific activity of ≥4.350× 10⁵ units/mg.

• 
  Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 1.136 ng/mL, corresponding to a specific activity is 8.803×10⁵ U/mg

Human

CHO

Tag Free

P01588 (A28-R193)

2056  [NCBI]

APPRLICDSRVLERYLLEAKEAENITTGCAEHCSLNENITVPDTKVNFYAWKRMEVGQQAVEVWQGLALLSEAVLRGQALLVNSSQPWEPLQLHVDKAVSGLRSLTTLLRALGAQKEAISPPDAASAAPLRTITADTFRKLFRVYSNFLRGKLKLYTGEACRTGDR

The protein migrates as an approximately 26-38 kDa band under reducing SDS-PAGE due to glycosylation.

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Calvillo L, et al. Recombinant human erythropoietin protects the myocardium from ischemia-reperfusion injury and promotes beneficial remodeling. Proc Natl Acad Sci U S A. 2003 Apr 15;100(8):4802-6.  [Content Brief]