1. 重组蛋白
  2. Receptor Proteins
  3. Cell Adhesion Molecules (CAMs)
  4. Immunoglobulin-like Cell Adhesion Molecules
  5. Endothelial Cell-Selective Adhesion Molecule (ESAM)
  6. ESAM 蛋白, Rat (HEK293, Fc)

ESAM 蛋白, Rat (HEK293, Fc)

目录号: HY-P74165
COA 产品使用指南

ESAM 蛋白通过同质分子相互作用促进聚集,并与 MAGI1 相互作用,增强其在细胞过程中的作用。ESAM 蛋白, Rat (HEK293, Fc) 是重组的 ESAM 蛋白,由 HEK293 表达,带有 C-hFc 标签。ESAM 蛋白, Rat (HEK293, Fc) 全长 222 个氨基酸,分子量为 57-70 kDa。

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     可免费申领三个不同产品的试用装。

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10 μg ¥800 In-stock
50 μg ¥2250 In-stock
100 μg ¥3800 In-stock
> 100 μg   询价  

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ESAM Protein, Rat (HEK293, Fc) 相关产品:

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

ESAM protein promotes aggregation through a homophilic molecular interaction and interacts with MAGI1, enhancing its role in cellular processes. ESAM Protein, Rat (HEK293, Fc) is the recombinant rat-derived ESAM protein, expressed by HEK293 , with C-hFc labeled tag. The total length of ESAM Protein, Rat (HEK293, Fc) is 222 a.a., with molecular weight of 57-70 kDa.

研究背景

ESAM 蛋白具有促进聚集的能力,主要是通过亲同性分子相互作用。它还与 MAGI1 相互作用,从而有助于其在细胞过程中的功能作用。

生物活性

Measured in a cell proliferation assay using MDA-MB-231 cells. The ED50 for this effect is 40.66 ng/mL. Corresponding to a specific activity is 2.459×104 Unit/mg.

种属

Rat

表达系统

HEK293

标签

C-hFc

蛋白编号

Q6AYD4 (Q30-A251)

基因 ID

300519  [NCBI]

蛋白结构
N-term
ESAM (Q30-A251)
Accession # Q6AYD4
hFc
C-term
同用名
Endothelial Cell-Selective Adhesion Molecule; ESAM
氨基酸序列

QMELHVPPGLNKLEAVEGEEVVLPAWYTMAREESSSHPWEAPFLIWFLEQEGKEPKQVLSYFKGSLSNKPGVTLVHSISTRNVSLRLDALQEGDSGTYRCSVNAYDSDGKNIGHSIKTIELKVLVPPAPPSCSFQGVPYVGTNVTLNCKSPRSKPTAQYQWERLAPSSQVFFGPALDTVRGSLKLTNISTAMSGVYVCKAQNRVGFAQCNVTLDVMTGSKAA

分子量

Approximately 57-70 kDa due to the glycosylation

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
ESAM Protein, Rat (HEK293, Fc)
目录号:
HY-P74165
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