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  3. ESM-1 蛋白, Human (HEK293, His)

ESM-1 蛋白, Human (HEK293, His)

目录号: HY-P70151
COA 产品使用指南

ESM-1 (Endocan) 通过促进新血管萌芽,在血管生成中发挥重要作用。它在肺内皮细胞-白细胞相互作用中的意义表明在调节肺血管系统内的免疫反应方面具有潜在意义。ESM-1 蛋白, Human (HEK293, His) 是重组的 ESM-1 蛋白,由 HEK293 表达,带有 C-6*His 标签。

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规格 价格 是否有货 数量
10 μg ¥1250 In-stock
50 μg ¥3750 In-stock
100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

ESM-1 (Endocan) serves a vital role in angiogenesis by promoting new blood vessel sprouting. Its implication in lung endothelial cell-leukocyte interactions suggests potential significance in regulating immune responses within the pulmonary vasculature. ESM-1 Protein, Human (HEK293, His) is the recombinant human-derived ESM-1 protein, expressed by HEK293 , with C-6*His labeled tag.

研究背景

ESM-1 (Endocan) 在血管生成中发挥着至关重要的作用,促进新血管的萌芽。它涉及肺内皮细胞-白细胞相互作用,表明其在调节肺血管系统内的免疫反应中具有潜在意义。

生物活性

Measured by its ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. The ED 50 for this effect is ≤1.615 μg/mL, corresponding to a specific activity is ≥6.19×102 units/mg.

  • Measured by its ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. The ED50 for this effect is 1.615 μg/mL, corresponding to a specific activity is 6.19×103 units/mg.
种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

AAH11989.1/Q9NQ30-1 (W20-R184 )

基因 ID
蛋白结构
N-term
ESM-1 (W20-R184 )
Accession # AAH11989.1
6*His
C-term
同用名
rHuEndothelial cell-specific molecule 1/ESM-1, His; Endothelial Cell-Specific Molecule 1; ESM-1; ESM1
氨基酸序列

WSNNYAVDCPQHCDSSECKSSPRCERTVLDDCGCCRVCAAGRGETCYRTVSGMDGMKCGPGLRCQPSNGEDPFGEEFGICKDCPYGTFGMDCRETCNCQSGICDRGTGKCLKFPFFQYSVTKSSNRFVSLTEHDMASGDGNIVREEVVKENAAGSPVMRKWLNPR

分子量

Approximately 20-26 kDa due to the glycosylation

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

ESM-1 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
ESM-1 Protein, Human (HEK293, His)
目录号:
HY-P70151
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