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  3. ETFR-2/TEAD-4 蛋白, Mouse

ETFR-2/TEAD-4 蛋白, Mouse

目录号: HY-P71575
COA 产品使用指南

ETFR-2/TEAD-4 蛋白是严格调节 Hippo 信号通路的关键转录因子,是器官大小控制和肿瘤抑制的重要组成部分。在此通路中,它介导涉及 MST1/MST2、SAV1、LATS1/2 和 MOB1 的激酶级联,导致 YAP1 和 WWTR1/TAZ 失活。ETFR-2/TEAD-4 蛋白, Mouse 是重组的 ETFR-2/TEAD-4 蛋白,由 E. coli 表达,不带标签。ETFR-2/TEAD-4 蛋白, Mouse 全长 218 个氨基酸,分子量约为 ~25.7 kDa。

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规格 价格 是否有货 数量
20 μg ¥3000 In-stock
50 μg ¥6600 In-stock
100 μg   询价  

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描述

ETFR-2/TEAD-4 protein is a key transcription factor that tightly regulates the Hippo signaling pathway and is an integral component of organ size control and tumor suppression. In this pathway, it mediates a kinase cascade involving MST1/MST2, SAV1, LATS1/2, and MOB1, leading to the inactivation of YAP1 and WWTR1/TAZ. ETFR-2/TEAD-4 Protein, Mouse is the recombinant mouse-derived ETFR-2/TEAD-4 protein, expressed by E. coli , with tag free. The total length of ETFR-2/TEAD-4 Protein, Mouse is 218 a.a., with molecular weight of ~25.7 kDa.

研究背景

ETFR-2/TEAD-4 是一种关键的转录因子,在 Hippo 信号通路中发挥着关键作用,该通路通过增殖和凋亡的调节与器官大小控制和肿瘤抑制密切相关。该途径涉及激酶级联,其中 MST1/MST2 与其调节蛋白 SAV1 复合,磷酸化并激活与其调节蛋白 MOB1 复合的 LATS1/2。随后,MOB1 磷酸化并灭活 YAP1 癌蛋白和 WWTR1/TAZ。ETFR-2/TEAD-4 通过介导 YAP1 和 WWTR1/TAZ 的基因表达发挥作用,从而调节关键的细胞过程,例如增殖、迁移和上皮间质转化 (EMT) 诱导。该蛋白与 Sph 和 GT-IIC“enhansons”(5'-GTGGAATGT-3') 特异性且非合作性结合并激活转录。此外,它还与 M-CAT 基序相互作用。虽然 ETFR-2/TEAD-4 可能在骨骼肌的胚胎发育中发挥作用,但它还与 WWTR1/TAZ 和 YAP1 相互作用,突出了其在调控网络中的多方面参与。

种属

Mouse

表达系统

E. coli

标签

Tag Free

蛋白编号

Q62296 (210R-427E)

基因 ID

21679  [NCBI]

蛋白结构
N-term
ETFR-2 (210R-427E)
Accession # Q62296
C-term
同用名
ETF-related factor 2; ETFR-2; TEA domain family member 4; TEAD-4; TEF-1-related factor 1; TEF-1-related factor FR-19; RTEF-1
氨基酸序列

RSIASSKLWMLEFSAFLERQQDPDTYNKHLFVHISQSSPSYSDPYLETVDIRQIYDKFPEKKGGLKELFERGPSNAFFLVKFWADLNTNIDDEGSAFYGVSSQYESPENMIITCSTKVCSFGKQVVEKVETEYARYENGHYLYRIHRSPLCEYMINFIHKLKHLPEKYMMNSVLENFTILQVVTNRDTQETLLCIAYVFEVSASEHGAQHHIYRLVKE

分子量

Approximately 25.7 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

无菌粉末

组分

Lyophilized after extensive dialysis against solution in 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

ETFR-2/TEAD-4 Protein, Mouse 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
ETFR-2/TEAD-4 Protein, Mouse
目录号:
HY-P71575
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