ETHE1 蛋白, Human (His)

The ETHE1 protein is an important sulfur dioxygenase that plays an integral role in mitochondrial hydrogen sulfide catabolism. It cooperates with SQRDL to oxidize cysteine persulfide residues formed from hydrogen sulfide (H2S), preventing the accumulation of toxic H2S and maintaining metabolic homeostasis. ETHE1 Protein, Human (His) is the recombinant human-derived ETHE1 protein, expressed by E. coli , with N-His labeled tag. The total length of ETHE1 Protein, Human (His) is 242 a.a., with molecular weight of ~28 KDa.

ETHE1 蛋白是一种重要的硫双加氧酶，在线粒体基质内的硫化氢分解代谢中发挥着不可或缺的作用。在此过程中，硫化氢 (H(2)S) 首先被 SQRDL 氧化，形成半胱氨酸过硫化物残基。一旦过硫化物转移到亲硫受体（如谷胱甘肽 (R-SSH)）上，ETHE1 利用分子氧催化随后的氧化。这种酶活性对于维持线粒体代谢稳态至关重要，防止超生理 H(2)S 水平的积累，而超生理 H(2)S 水平可能因细胞色素 c 氧化酶的抑制而产生毒性。除了在硫化氢代谢中的作用之外，ETHE1 最初被认为是一种能够在细胞核和细胞质之间穿梭的蛋白质。它通过将转录因子 RELA/NFKB3 隔离在细胞质中，防止其在细胞核中积累，从而抑制 p53 诱导的细胞凋亡。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-His

O95571 (L13-A254)

23474  [NCBI]

LSQRGGSGAPILLRQMFEPVSCTFTYLLGDRESREAVLIDPVLETAPRDAQLIKELGLRLLYAVNTHCHADHITGSGLLRSLLPGCQSVISRLSGAQADLHIEDGDSIRFGRFALETRASPGHTPGCVTFVLNDHSMAFTGDALLIRGCGRTDFQQGCAKTLYHSVHEKIFTLPGDCLIYPAHDYHGFTVSTVEEERTLNPRLTLSCEEFVKIMGNLNLPKPQQIDFAVPANMRCGVQTPTA

Approximately 28 kDa.

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in PBS. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.