FANCC 蛋白, Human (His-SUMO)

FANCC Protein repairs and maintains DNA, aids in STAT1 activation, and forms a multisubunit FA complex. It interacts with ZBTB32, STAT1, CDK1, and EIF2AK2. The FA complex excludes FA patients and may include HSP70, while interaction with EIF2AK2 leads to increased activation and cell death. FANCC Protein, Human (His-SUMO) is the recombinant human-derived FANCC protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag. The total length of FANCC Protein, Human (His-SUMO) is 558 a.a., with molecular weight of ~79.4 kDa.

FANCC 蛋白是范可尼贫血 (FA) 复合物的重要组成部分，与 DNA 修复有关，可能在复制后修复或细胞周期检查点内发挥作用。它可能在DNA链间交联的修复中发挥至关重要的作用，并有助于维持正常的染色体稳定性。在干扰素-γ (IFNG) 诱导后，FANCC 可能通过将 STAT1 招募到 IFNGR1 来促进 STAT1 的激活。作为多亚基 FA 复合体（由 FANCA、FANCB、FANCC、FANCE、FANCF、FANCG、FANCL/PHF9 和 FANCM 组成）的一部分，它可能包含 HSP70。重要的是，范可尼贫血患者不存在这种复合物。FANCC 与多种蛋白质相互作用，包括 ZBTB32、STAT1（尤其是在 IFNG 诱导后）、CDK1 和 EIF2AK2。FANCC 变体和 EIF2AK2 之间的相互作用可能导致 EIF2AK2 激活增强和随后的细胞死亡。这些相互作用强调了 FANCC 在与 DNA 修复、信号传导和基因组稳定性维持相关的细胞过程中的多方面作用。

Human

E. coli

N-6*His;N-SUMO

Q00597 (M1-V558)

2176  [NCBI]

MAQDSVDLSCDYQFWMQKLSVWDQASTLETQQDTCLHVAQFQEFLRKMYEALKEMDSNTVIERFPTIGQLLAKACWNPFILAYDESQKILIWCLCCLINKEPQNSGQSKLNSWIQGVLSHILSALRFDKEVALFTQGLGYAPIDYYPGLLKNMVLSLASELRENHLNGFNTQRRMAPERVASLSRVCVPLITLTDVDPLVEALLICHGREPQEILQPEFFEAVNEAILLKKISLPMSAVVCLWLRHLPSLEKAMLHLFEKLISSERNCLRRIECFIKDSSLPQAACHPAIFRVVDEMFRCALLETDGALEIIATIQVFTQCFVEALEKASKQLRFALKTYFPYTSPSLAMVLLQDPQDIPRGHWLQTLKHISELLREAVEDQTHGSCGGPFESWFLFIHFGGWAEMVAEQLLMSAAEPPTALLWLLAFYYGPRDGRQQRAQTMVQVKAVLGHLLAMSRSSSLSAQDLQTVAGQGTDTDLRAPAQQLIRHLLLNFLLWAPGGHTIAWDVITLMAHTAEITHEIIGFLDQTLYRWNRLGIESPRSEKLARELLKELRTQV

Approximately 79.4 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm solution of Tris-based buffer, 50% Glycerol.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.