1. 重组蛋白
  2. Immune Checkpoint Proteins CD Antigens Fc Receptors Biotinylated Proteins
  3. Inhibitory Checkpoint Molecules T Cell CD Proteins NK Cell CD Proteins Macrophage CD Proteins Monocyte CD Proteins Dendritic Cell CD Proteins Fc-gamma Receptor
  4. FcγRIIIA/CD16a CD300a Fc gamma RIII/CD16
  5. Fc gamma RIIIA/CD16a 蛋白, Human (Biotinylated, HEK293, His-Avi)

Fc gamma RIIIA/CD16a 蛋白, Human (Biotinylated, HEK293, His-Avi)

目录号: HY-P78099
COA 产品使用指南

Fc gamma RIIIA/CD16a 蛋白是 IgG Fc 片段的受体,在与抗原-IgG 复合物结合后激活抗体依赖性细胞毒性 (ADCC)。它介导 NK 细胞上的 IgG 效应器功能,产生类似记忆的适应性 NK 细胞,以有效消除病毒感染的细胞。Fc gamma RIIIA/CD16a 蛋白, Human (Biotinylated, HEK293, His-Avi) 是重组的 Fc gamma RIIIA/CD16a 蛋白,由 HEK293 表达,带有 C-Avi, C-His 标签。Fc gamma RIIIA/CD16a 蛋白, Human (Biotinylated, HEK293, His-Avi) 全长 192 个氨基酸,分子量为 48-60 kDa。

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规格 价格 是否有货 数量
20 μg ¥2450 In-stock
50 μg ¥5400 In-stock
100 μg ¥9180 询价
> 100 μg   询价  

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描述

Fc gamma RIIIA/CD16a protein is a receptor for the Fc fragment of IgG and activates antibody-dependent cellular cytotoxicity (ADCC) upon binding to antigen-IgG complexes. It mediates IgG effector functions on NK cells, generating memory-like adaptive NK cells to efficiently eliminate virally infected cells. Fc gamma RIIIA/CD16a Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived Fc gamma RIIIA/CD16a protein, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of Fc gamma RIIIA/CD16a Protein, Human (Biotinylated, HEK293, His-Avi) is 192 a.a., with molecular weight of 48-60 kDa.

研究背景

Fc gamma RIIIA/CD16a 蛋白作为免疫球蛋白 gamma (IgG) 不变 Fc 片段的受体,在与细胞表面展示的聚集的抗原-IgG 复合物结合时最佳激活,启动抗体依赖性细胞毒性 (ADCC)。该过程涉及抗体包被细胞的裂解,防止在没有抗原触发的情况下效应细胞的不适当激活。该蛋白介导自然杀伤 (NK) 细胞上的 IgG 效应器功能,结合感染过程中产生的抗原-IgG 复合物,触发 NK 细胞依赖性细胞因子的产生和脱颗粒。Fc gamma RIIIA/CD16a 对于生成类似记忆的适应性 NK 细胞至关重要,这些细胞可通过 ADCC 有效消除病毒感染的细胞。它调节 NK 细胞的存活、增殖,并防止 NK 细胞祖细胞凋亡。作为 Fc 结合亚基,它与 CD247 和/或 FCER1G 接头结合形成功能性信号传导复合物,导致驱动 NK 细胞激活的细胞内信号传导级联。该蛋白还在介导治疗性抗体的抗肿瘤活性、触发 IgG 包被肿瘤细胞的 TNFA 依赖性 ADCC 以及增强对无岩藻糖基化 IgG 的 ADCC 反应方面发挥作用。在登革热病毒感染的情况下,FcγRIIIA/CD16a通过抗体依赖性增强(ADE)机制参与发病机制,促进病毒进入骨髓细胞并随后在继发感染期间进行病毒复制。

生物活性

Measured by its binding ability in a functional ELISA. When immobilized Anti-Fc gamma RIIIA Antibody, hFc Tag at 1μg/ml (100μl/Well), can bind Biotinylated Human Fc gamma RIIIA (F176), His Tag and the EC50 is 79.2ng/ml.

种属

Human

表达系统

HEK293

标签

C-Avi;C-His

蛋白编号

P08637 (G17-Q208)

基因 ID
蛋白结构
N-term
CD16a (G17-Q208)
Accession # P08637
His-Avi
C-term
同用名
CD16FCRIIIA; Fc fragment of IgG; Fc gamma RIIIA; Fc-gamma RIII-alpha; FCGR3; FCGR3A; FCGRIII; FcgRIIIA; FcR-10; CD16A; FCG3; IGFR3; FcRIIIa; FcRIII; CD16; IMD20
分子量

48-60 kDa

纯度

Greater than 95% as determined by Tris-Bis PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Fc gamma RIIIA/CD16a Protein, Human (Biotinylated, HEK293, His-Avi)
目录号:
HY-P78099
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