2. 重组蛋白
  3. Enzymes & Regulators
  4. Oxidoreductases (EC 1)
  5. FDH1 蛋白, Candida boidinii

FDH1 蛋白, Candida boidinii

目录号: HY-P702119
产品使用指南

FDH1 蛋白在依赖 NAD(+) 的甲酸氧化成二氧化碳的过程中充当催化剂。这种酶活性代表了甲基营养型微生物中甲醇氧化途径的决定性步骤。除了参与甲基营养生物体之外，FDH1 在非甲基营养生物体中也发挥着至关重要的作用，它有助于外源甲酸的解毒。FDH1 蛋白, Candida boidinii 是重组的 FDH1 蛋白，由 E. coli 表达，不带标签。FDH1 蛋白, Candida boidinii 全长 364 个氨基酸。

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描述

FDH1 protein serves as a catalyst in the NAD(+)-dependent oxidation of formate to carbon dioxide. This enzymatic activity represents the conclusive step in the methanol oxidation pathway within methylotrophic microorganisms. Beyond its involvement in methylotrophic organisms, FDH1 also plays a crucial role in non-methylotrophic organisms, where it contributes to the detoxification of exogenous formate. FDH1 Protein, Candida boidinii is the recombinant FDH1 protein, expressed by E. coli , with tag free. The total length of FDH1 Protein, Candida boidinii is 364 a.a., .
研究背景

FDH1 蛋白是一种酶，通过催化 NAD(+) 依赖性甲酸氧化成二氧化碳，在甲基营养微生物中发挥着至关重要的作用。这种酶活性是甲醇氧化途径的最后一步。此外，FDH1 在非甲基营养生物中具有重要作用，它参与外源甲酸的解毒，有助于细胞稳态并防止潜在有害甲酸水平的积累。
种属

Others
表达系统

E. coli
标签

Tag Free
蛋白编号

O13437 (M1-K364)
基因 ID

/
蛋白结构
N-term
FDH1 (M1-K364)
Accession # O13437
C-term
同用名
FDH1; Formate dehydrogenase; FDH; NAD-dependent formate dehydrogenase
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

FDH1 Protein, Candida boidinii 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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