Fetuin B 蛋白, Human (367a.a, HEK293, His)

Fetuin B, a vital protease inhibitor, plays a crucial role in egg fertilization by preventing premature zona pellucida hardening before fertilization. Its primary function involves inhibiting the protease activity of ASTL, preventing ZP2 cleavage and zona pellucida hardening. Fetuin B's regulatory role emphasizes its significance in creating optimal conditions for successful fertilization and subsequent embryonic development. Fetuin B Protein, Human (367a.a, HEK293, His) is the recombinant human-derived Fetuin B protein, expressed by HEK293, with C-6*His labeled tag. The total length of Fetuin B Protein, Human (367a.a, HEK293, His) is 367 a.a., with molecular weight of 53-55 kDa.

胎球蛋白 B 是卵子受精所必需的重要蛋白酶抑制剂。它的主要作用是防止受精前透明带过早硬化，这是成功胚胎发育的关键过程。这一功能可能是通过抑制 ASTL 的蛋白酶活性来介导的，ASTL 是一种负责裂解 ZP2 并引发透明带硬化的关键酶。胎球蛋白 B 在此过程中的调节作用凸显了其在促进成功受精和随后胚胎发育的适当条件方面的重要性。

Measured by its ability to inhibit active Cathepsin V cleavage of a fluorogenic peptide substrate Z-Leu-Arg-AMC. The IC₅₀ value is 21.16 nM, as measured under the described conditions.

Human

HEK293

C-6*His

Q9UGM5 (C16-P382)

26998  [NCBI]

CGAMSPPQLALNPSALLSRGCNDSDVLAVAGFALRDINKDRKDGYVLRLNRVNDAQEYRRGGLGSLFYLTLDVLETDCHVLRKKAWQDCGMRIFFESVYGQCKAIFYMNNPSRVLYLAAYNCTLRPVSKKKIYMTCPDCPSSIPTDSSNHQVLEAATESLAKYNNENTSKQYSLFKVTRASSQWVVGPSYFVEYLIKESPCTKSQASSCSLQSSDSVPVGLCKGSLTRTHWEKFVSVTCDFFESQAPATGSENSAVNQKPTNLPKVEESQQKNTPPTDSPSKAGPRGSVQYLPDLDDKNSQEKGPQEAFPVHLDLTTNPQGETLDISFLFLEPMEEKLVVLPFPKEKARTAECPGPAQNASPLVLPP

53-55 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2 or PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.