FGF-1 蛋白, Human (154a.a)

FGF-1 Protein intricately regulates cell survival, division, angiogenesis, differentiation, and migration. Acting as a potent mitogen, it serves as a ligand for FGFR1 and integrins. Binding to FGFR1, especially with heparin, leads to dimerization and activation, initiating signaling cascades. Additionally, FGF-1 Protein binds to integrin ITGAV:ITGB3, forming a ternary complex crucial for FGF1 signaling. Inducing phosphorylation of key proteins, it plays a multifaceted role in diverse cellular processes, emphasizing its significance in cell physiology. FGF-1 Protein, Human (154a.a) is the recombinant human-derived FGF-1 protein, expressed by E. coli, with His, tag free. The total length of FGF-1 Protein, Human (154a.a) is 154 a.a., with molecular weight of 18.54 kDa.

FGF-1 蛋白在复杂的细胞存活、分裂、血管生成、分化和迁移调节中发挥着关键作用。它在体外充当有效的有丝分裂原，充当 FGFR1 和整合素的配体。与 FGFR1 结合，特别是在存在肝素的情况下，会导致 FGFR1 二聚化，并通过酪氨酸残基上的连续自磷酸化来激活。这种激活充当相互作用蛋白质的对接位点，启动多个信号级联反应。此外，FGF-1 蛋白与整合素 ITGAV:ITGB3 结合，与整合素和 FGFR1 形成三元复合物，这对 FGF1 信号转导至关重要。它诱导 FGFR1、FRS2、MAPK3/ERK1、MAPK1/ERK2 和 AKT1 的磷酸化和激活，证明其参与多种细胞过程。此外，FGF-1 蛋白诱导血管生成的能力进一步强调了其多方面的作用。复杂网络中与 FGFR、整合素和各种蛋白质的相互作用凸显了其在细胞信号传导途径中的复杂参与，强调了其在细胞生理学中的重要性。

Human

E. coli

Tag Free

P05230 (A2-D155)

2246  [NCBI]

AEGEITTFTALTEKFNLPPGNYKKPKLLYCSNGGHFLRILPDGTVDGTRDRSDQHIQLQLSAESVGEVYIKSTETGQYLAMDTDGLLYGSQTPNEECLFLERLEENHYNTYISKKHAEKNWFVGLKKNGSCKRGPRTHYGQKAILFLPLPVSSD

18.54 KDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.