FGF-18 蛋白, Human

FGF-18 Protein, Human is a heparin-binding polypeptide growth factor, involved in cartilage growth, maturation and the development of functional cartilage and bone tissue.

成纤维细胞生长因子 18 (FGF18) 是一种分泌型肝素结合多肽生长因子，与 FGF8 和 175 相关，并已被证明在不同器官中具有多种功能。FGF18 参与软骨生长和成熟，并参与功能性软骨和骨组织的发育。它还参与成熟软骨10-13 内的过程，并被证明具有增强再生和修复的作用^[1]。成纤维细胞生长因子 18 (FGF18) 在骨骼生长和肢体发育中发挥重要作用，可能通过调节成骨细胞、软骨细胞和破骨细胞^[2]。

1.The ED₅₀ is <10 ng/mL as measured by 3T3 cells, corresponding to a specific activity of > 1.0 × 10⁵ units/mg.
2.Measured by its binding ability in a functional ELISA. Immobilized Human FGF18 at 10 μg/mL (100 μL/well) can bind Biotinylated Rat FGFR4 protein. The ED₅₀ for this effect is 0.8084 μg/mL.

• 
  Measured by its binding ability in a functional ELISA. Immobilized Human FGF18 at 10 μg/mL (100 μL/well) can bind Biotinylated Rat FGFR4 protein. The ED50 for this effect is 0.8084 μg/mL.

Human

E. coli

Tag Free

O76093 (A27-R199)

8817  [NCBI]

AEENVDFRIHVENQTRARDDVSRKQLRLYQLYSRTSGKHIQVLGRRISARGEDGDKYAQLLVETDTFGSQVRIKGKETEFYLCMNRKGKLVGKPDGTSKECVFIEKVLENNYTALMSAKYSGWYVGFTKKGRPRKGPKTRENQQDVHFMKRYPKGQPELQKPFKYTTVTKRSR

Approximately 20.3 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS or 20 mM PB, 150 mM NaCl, pH 7.4.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Barr L, et al. The effect of recombinant human fibroblast growth factor-18 on articular cartilage following single impact load. J Orthop Res. 2014 Jul;32(7):923-7.  [Content Brief]

  [2]. Chen T, et al. Fibroblast growth factor 18 promotes proliferation and migration of H460 cells via the ERK and p38 signaling pathways. Oncol Rep. 2017 Feb;37(2):1235-1242.  [Content Brief]