1. 重组蛋白
  2. Cytokines and Growth Factors
  3. FGF Family
  4. Fibroblast Growth Factor
  5. FGF-9
  6. FGF-9 蛋白, Human (HEK293, Fc, solution)

FGF-9 蛋白, Human (HEK293, Fc, solution)

目录号: HY-P73053
COA 产品使用指南

Activin AB 蛋白是转化生长因子-β (TGF-β) 超家族的成员,在细胞分化、增殖和凋亡等多种生物过程中发挥关键作用。它参与胚胎发育、组织修复和免疫调节。FGF-9 蛋白, Human (HEK293, Fc, solution) 是重组的 FGF-9 蛋白,由 HEK293 表达,带有 N-hFc 标签。FGF-9 蛋白, Human (HEK293, Fc, solution) 全长 205 个氨基酸,分子量分别约为 ~54 & 37 kDa。

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规格 价格 是否有货 数量
Free Sample   Apply now  
2 μg ¥700 In-stock
5 μg ¥1250 In-stock
10 μg ¥1960 In-stock
20 μg ¥3000 询价
50 μg ¥5500 In-stock
100 μg   询价  

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描述

Activin AB protein is a member of the transforming growth factor-β (TGF-β) superfamily and plays a key role in various biological processes such as cell differentiation, proliferation, and apoptosis. It is involved in embryonic development, tissue repair and immune regulation. FGF-9 Protein, Human (HEK293, Fc, solution) is the recombinant human-derived FGF-9 protein, expressed by HEK293 , with N-hFc labeled tag. The total length of FGF-9 Protein, Human (HEK293, Fc, solution) is 205 a.a., with molecular weight of ~54 & 37 kDa, respectively.

研究背景

FGF-9蛋白属于成纤维细胞生长因子(FGF)家族,具有广泛的有丝分裂和细胞存活活性,参与胚胎发育、细胞生长、形态发生、组织修复、肿瘤生长和侵袭等多种生物过程。这种蛋白质最初被认为是一种对培养的神经胶质细胞具有生长刺激作用的分泌因子,主要由神经系统中的神经元产生,这表明它在神经胶质细胞发育中的重要性。FGF-9 的小鼠同源物的表达取决于 Sonic Hedgehog (Shh) 信号传导,小鼠中 FGF-9 的缺失会导致雄性到雌性的性别逆转表型,表明其在睾丸胚胎发生中的作用。值得注意的是 FGF-9 的偏向表达,在肾脏 (RPKM 6.4)、肾上腺 (RPKM 2.1) 和 10 个其他组织中检测到水平升高,强调了其在多个器官的各种生理环境中的潜在重要性。

生物活性

Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblasts and the ED50 is typically 10-60 ng/mL.

种属

Human

表达系统

HEK293

标签

N-hFc

蛋白编号

NP_002001.1 (L4-S208)

基因 ID
蛋白结构
N-term
hFc
FGF-9 (L4-S208)
Accession # NP_002001.1
C-term
同用名
Fibroblast growth factor 9; FGF-9; GAF; HBGF-9
氨基酸序列

LGEVGNYFGVQDAVPFGNVPVLPVDSPVLLSDHLGQSEAGGLPRGPAVTDLDHLKGILRRRQLYCRTGFHLEIFPNGTIQGTRKDHSRFGILEFISIAVGLVSIRGVDSGLYLGMNEKGELYGSEKLTQECVFREQFEENWYNTYSSNLYKHVDTGRRYYVALNKDGTPREGTRTKRHQKFTHFLPRPVDPDKVPELYKDILSQS

分子量

Approximately 54&37 kDa

纯度

Greater than 85%, as determined by reducing SDS-PAGE

性状

溶液

组分

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

N/A.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料

FGF-9 Protein, Human (HEK293, Fc, solution) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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