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  3. Fibronectin 蛋白, Human

Fibronectin 蛋白, Human

目录号: HY-P70593
COA 产品使用指南

纤连蛋白结合胶原蛋白、纤维蛋白、肝素、DNA 和肌动蛋白。它参与细胞粘附、运动、调理作用、伤口愈合和细胞形状的维持。Fibronectin 蛋白, Human 是重组的蛋白二聚体复合物,包含 纤连蛋白,由 E. coli 表达,不带标签。Fibronectin 蛋白, Human ,分子量为 60-74 kDa。

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5 μg ¥350 In-stock
10 μg ¥500 In-stock
50 μg ¥1250 In-stock
100 μg ¥2000 询价
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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

Fibronectin binds collagen, fibrin, heparin, DNA, and actin. It is involved in cell adhesion, motility, opsonization, wound healing and maintenance of cell shape. Fibronectin Protein, Human is a recombinant protein dimer complex containing human-derived Fibronectin protein, expressed by E. coli , with tag free. Fibronectin Protein, Human, has molecular weight of 60-74 kDa.

研究背景

纤连蛋白是一种多功能糖蛋白,通过与胶原蛋白、纤维蛋白、肝素、DNA 和肌动蛋白等多种化合物结合,在细胞相互作用中发挥着至关重要的作用。它参与细胞粘附、运动、调理作用、伤口愈合和细胞形状的维持,强调了它在基本细胞过程中的重要性。纤连蛋白还通过纤连蛋白原纤维形成细胞介导的基质组装过程促进成骨细胞压实,这对成骨细胞矿化至关重要,并调节成骨细胞沉积 I 型胶原。作为 LILRB4 受体的配体,纤连蛋白抑制 FCGR1A/CD64 介导的单核细胞活化。此外,如在名为超纤连蛋白的纤连蛋白聚合物中所观察到的,纤连蛋白诱导原纤维形成的能力赋予增强的粘合性能。值得注意的是,阿纳斯特林和超纤连蛋白均对肿瘤生长、血管生成和转移具有抑制作用,阿纳斯特林激活 p38 MAPK 并抑制溶血磷脂信号传导。纤连蛋白的多方面功能强调了其在细胞动力学中的关键作用及其作为各种病理状况的治疗干预目标的潜力。

生物活性

1.Measured by its ability to support cell attachment and spreading when used as a substratum for cell culture. The ED50 for this effect is ≤ 385.7 ng/mL.
2.Measured by the ability of the immobilized protein to support the adhesion of B16-F1 mouse melanoma cells. The ED50 for this effect is 124.5 ng/mL, corresponding to a specific activity is 8.03×103 units/mg.

  • Measured by the ability of the immobilized protein to support the adhesion of B16-F1 mouse melanoma cells. The ED50 for this effect is 124.5 ng/mL, corresponding to a specific activity is 8.03×103 units/mg.
种属

Human

表达系统

E. coli

标签

Tag Free

蛋白编号

P02751-1 (P1270-S1546 & A1721-T2016)

基因 ID
蛋白结构
N-term
Fibronectin (P1270-S1546 & A1721-T2016)
Accession # P02751-1
C-term
同用名
NovoNectin; Fibronectin; FN; Cold-insoluble globulin; CIG; FN; Fibronectin 1
氨基酸序列

PTDLRFTNIGPDTMRVTWAPPPSIDLTNFLVRYSPVKNEEDVAELSISPSDNAVVLTNLLPGTEYVVSVSSVYEQHESTPLRGRQKTGLDSPTGIDFSDITANSFTVHWIAPRATITGYRIRHHPEHFSGRPREDRVPHSRNSITLTNLTPGTEYVVSIVALNGREESPLLIGQQSTVSDVPRDLEVVAATPTSLLISWDAPAVTVRYYRITYGETGGNSPVQEFTVPGSKSTATISGLKPGVDYTITVYAVTGRGDSPASSKPISINYRTEIDKPS&AIPAPTDLKFTQVTPTSLSAQWTPPNVQLTGYRVRVTPKEKTGPMKEINLAPDSSSVVVSGLMVATKYEVSVYALKDTLTSRPAQGVVTTLENVSPPRRARVTDATETTITISWRTKTETITGFQVDAVPANGQTPIQRTIKPDVRSYTITGLQPGTDYKIYLYTLNDNARSSPVVIDASTAIDAPSNLRFLATTPNSLLVSWQPPRARITGYIIKYEKPGSPPREVVPRPRPGVTEATITGLEPGTEYTIYVIALKNNQKSEPLIGRKKTDELPQLVTLPHPNLHGPEILDVPST

分子量

60-74 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of 12.5 mM Sodium Citrate, 1.25% Sucrose, pH 5.5.

内毒素含量

<0.01 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Fibronectin Protein, Human 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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