Fibronectin 蛋白, Human

Fibronectin binds collagen, fibrin, heparin, DNA, and actin. It is involved in cell adhesion, motility, opsonization, wound healing and maintenance of cell shape. Fibronectin Protein, Human is the recombinant human-derived Fibronectin protein, expressed by E. coli, with tag free.

纤连蛋白是一种多功能糖蛋白，通过与胶原蛋白、纤维蛋白、肝素、DNA 和肌动蛋白等多种化合物结合，在细胞相互作用中发挥着至关重要的作用。它参与细胞粘附、运动、调理作用、伤口愈合和细胞形状的维持，强调了它在基本细胞过程中的重要性。纤连蛋白还通过纤连蛋白原纤维形成细胞介导的基质组装过程促进成骨细胞压实，这对成骨细胞矿化至关重要，并调节成骨细胞沉积 I 型胶原。作为 LILRB4 受体的配体，纤连蛋白抑制 FCGR1A/CD64 介导的单核细胞活化。此外，如在名为超纤连蛋白的纤连蛋白聚合物中所观察到的，纤连蛋白诱导原纤维形成的能力赋予增强的粘合性能。值得注意的是，阿纳斯特林和超纤连蛋白均对肿瘤生长、血管生成和转移具有抑制作用，阿纳斯特林激活 p38 MAPK 并抑制溶血磷脂信号传导。纤连蛋白的多方面功能强调了其在细胞动力学中的关键作用及其作为各种病理状况的治疗干预目标的潜力。

Fibronectin 蛋白 (Human) (2-40 nM，24-100 h) 以剂量依赖地方式促进缺乏内源性纤维连接蛋白的小鼠胚胎细胞生长^[11]。
Fibronectin 蛋白 (Human) 对人类 LILRB4 受体和其在小鼠中的直系同源物 gp49B 受体具有较高的结合亲和力，K_D值分别为 421 nM 和 50.1 nM^[12]。

FN30-Fc 是由 Fibronectin 蛋白 (Human) 的 N 端 30 kDa (FN30) 与小鼠 IgG2a Fc 融合形成的重组蛋白。FN30-Fc (200 μg，10 周龄小鼠，腹腔注射，共 2 次，间隔 2 周) 在 BXSB/Yaa 红斑狼疮 (SLE) 小鼠模型具有改善其自身免疫性肾炎的作用^[12]。

1.Measured by its ability to support cell attachment and spreading when used as a substratum for cell culture. The ED₅₀ for this effect is ≤ 385.7 ng/mL.
2.Measured by the ability of the immobilized protein to support the adhesion of B16-F1 mouse melanoma cells. The ED₅₀ for this effect is ≤182.9 ng/mL, corresponding to a specific activity is ≥5.467×10³ units/mg.

• 
  Measured by the ability of the immobilized protein to support the adhesion of B16-F1 mouse melanoma cells. The ED₅₀ for this effect is 124.5 ng/mL, corresponding to a specific activity is 8.03×10³ units/mg.

Human

E. coli

Tag Free

P02751-1 (P1270-S1546&A1721-T2016)

2335  [NCBI]

Partial

PTDLRFTNIGPDTMRVTWAPPPSIDLTNFLVRYSPVKNEEDVAELSISPSDNAVVLTNLLPGTEYVVSVSSVYEQHESTPLRGRQKTGLDSPTGIDFSDITANSFTVHWIAPRATITGYRIRHHPEHFSGRPREDRVPHSRNSITLTNLTPGTEYVVSIVALNGREESPLLIGQQSTVSDVPRDLEVVAATPTSLLISWDAPAVTVRYYRITYGETGGNSPVQEFTVPGSKSTATISGLKPGVDYTITVYAVTGRGDSPASSKPISINYRTEIDKPS&AIPAPTDLKFTQVTPTSLSAQWTPPNVQLTGYRVRVTPKEKTGPMKEINLAPDSSSVVVSGLMVATKYEVSVYALKDTLTSRPAQGVVTTLENVSPPRRARVTDATETTITISWRTKTETITGFQVDAVPANGQTPIQRTIKPDVRSYTITGLQPGTDYKIYLYTLNDNARSSPVVIDASTAIDAPSNLRFLATTPNSLLVSWQPPRARITGYIIKYEKPGSPPREVVPRPRPGVTEATITGLEPGTEYTIYVIALKNNQKSEPLIGRKKTDELPQLVTLPHPNLHGPEILDVPST

Approximately 63 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 12.5 mM Sodium Citrate, 1.25% Sucrose, pH 5.5 or PBS, pH 6.8, 5% trehalose, 5% mannitol and 0.01% Tween 80.

<0.01 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Patten J, Wang K. Fibronectin in development and wound healing. Adv Drug Deliv Rev. 2021 Mar;170:353-368.  [Content Brief]

  [2]. Lenselink EA. Role of fibronectin in normal wound healing. Int Wound J. 2015 Jun;12(3):313-6. doi: 10.1111/iwj.12109. Epub 2013 Jun 7.  [Content Brief]

  [3]. Bielefeld K A, et al. Fibronectin and β-catenin act in a regulatory loop in dermal fibroblasts to modulate cutaneous healing[J]. Journal of Biological Chemistry, 2011, 286(31): 27687-27697.  [Content Brief]

  [4]. Shi F, et al. MT1-MMP regulates the turnover and endocytosis of extracellular matrix fibronectin[J]. Journal of cell science, 2011, 124(23): 4039-4050.  [Content Brief]

  [5]. Lefcort F, et al. Regulation of expression of fibronectin and its receptor, α5β1, during development and regeneration of peripheral nerve[J]. Development, 1992, 116(3): 767-782.

  [6]. Clark RA, et al. Blood vessel fibronectin increases in conjunction with endothelial cell proliferation and capillary ingrowth during wound healing. J Invest Dermatol. 1982 Nov;79(5):269-76.  [Content Brief]

  [7]. Oyama F, et al. Deregulation of alternative splicing of fibronectin pre-mRNA in malignant human liver tumors. J Biol Chem. 1989 Jun 25;264(18):10331-4.  [Content Brief]

  [8]. Yi M, Ruoslahti E. A fibronectin fragment inhibits tumor growth, angiogenesis, and metastasis. Proc Natl Acad Sci U S A. 2001 Jan 16;98(2):620-4.  [Content Brief]

  [9]. Quirós J, et al. Beneficial effect of fibronectin administration on chronic nephritis in rats. Arthritis Rheum. 1990 May;33(5):685-92.  [Content Brief]

  [10]. Su MT, et al. Blockade of checkpoint ILT3/LILRB4/gp49B binding to fibronectin ameliorates autoimmune disease in BXSB/Yaa mice. Int Immunol. 2021 Jul 23;33(8):447-458.  [Content Brief]

  [11]. Sottile J, et al. Fibronectin matrix assembly enhances adhesion-dependent cell growth. J Cell Sci. 1998 Oct;111 ( Pt 19):2933-43.  [Content Brief]