1. 重组蛋白
  2. Cytokines and Growth Factors
  3. TGF-beta Superfamily
  4. Activin/Inhibins Receptor
  5. Follistatin
  6. Follistatin/FST 蛋白, Human (HEK293, Fc)

Follistatin/FST 蛋白, Human (HEK293, Fc)

目录号: HY-P73068
COA 产品使用指南

Follistatin (FST) 是 TGFβ 家族信号传导的调节剂,通过选择性结合 TGFβ 家族配体并阻止配体与受体复合物结合而发挥作用。Follistatin 具有抑制促卵泡激素 (FSH) 的能力。Follistatin/FST 蛋白, Human (HEK293, Fc) 在 HEK293 细胞中表达产生,由 344 个氨基酸(M1-W344) 组成,在 C 末端带有 Fc 标签。

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描述

Follistatin (FST) is a regulator of TGFβ family signaling and acts by selectively binding to TGFβ family ligands and preventing ligand binding to the receptor complex. Follistatin has the ability to suppress the follicle stimulating hormone (FSH)[1][2]. Follistatin/FST Protein, Human (HEK293, Fc) is produced in HEK293 cells with a C-Terminal Fc-tag. It consists of 344 amino acids (M1-W344).

研究背景

卵泡抑素首先被描述为存在于卵巢卵泡液中的卵泡刺激激素抑制物质。卵泡抑素以低纳摩尔 (nM) 亲和力结合激活素 A 和肌生长抑制素,完全包围配体,封闭所有受体结合位点并与配体结合[1][2] .
成熟的人卵泡抑素与小鼠和大鼠卵泡抑素具有 97% 的氨基酸序列同一性。
卵泡抑素是一种 32-35-kDa 的糖蛋白,由四个结构域组成,包括一个 N 端结构域 (ND),后跟三个结构域卵泡抑素结构域(FSD1、FSD2 和 FSD3)。卵泡抑素的 C 端剪接可产生各种同工型,包括 FS288 和 FS315。卵泡抑素通过围绕配体形成几乎不可逆的非信号复合物并防止与 TGFβ 受体相互作用,从而中和 TGFβ 配体、肌生长抑制素和激活素 A。在人类中,编码卵泡抑素的基因位于染色体 5q11.2 上。卵泡抑素蛋白含有 TGF-β 结合位点,其中激活素、骨形态生成蛋白 (BMP) 和生长分化因子 (GDF) 以高亲和力结合,从而被中和。卵泡抑素的配体结合位点与这些配体的 I 型和 II 型受体结合位点重叠。卵泡抑素还含有肝素结合位点,细胞外基质中的蛋白聚糖可以结合该位点,因此卵泡抑素被认为结合细胞外基质。卵泡抑素有两种主要亚型:FST288(通过与硫酸肝素蛋白聚糖相互作用固定在细胞表面)和 FST315(循环中的主要形式)。这两种异构体由选择性剪接产生。315 同种型包括 27 个氨基酸的酸性 C 末端尾部,而 Follistatin 288 则没有。Follistatin 315 上的酸性尾部中和肝素结合位点,从而抑制 Follistatin 315 与细胞外基质的结合[1][2][3]< /sup>.
卵泡抑素作为一种肝源性蛋白质,受胰高血糖素与胰岛素比例的调节,表明与能量代谢有关。在人类中,FST 和激活素的异常表达与不孕症有关。卵泡抑素是性腺、垂体、妊娠膜、脉管系统和肝脏中的有效组织调节剂
[1]
[3]

体外研究
(In Vitro)

Recombinant mouse Follistatin (.2 μg/mL; 1-48 hours) significantly inhibits P21 mRNA expression, and increases Pcna and Cyclin D2 mRNA expression in CD49f positive cells (CD49f+ cells) isolated from male murine salivary glands[4].

体内研究
(In Vivo)

Recombinant mouse Follistatin (.5-1 μg; four does) is administered intranasally (5 μL) 1 h before each challenge in ovalbumin-sensitized BALB/c mice. Follistatin inhibits the allergen-specific Th2 immune response in mediastinal lymph nodes and mucus production in the lung[5].

生物活性

Measured by its ability to neutralize Activin A inhibitory effect of murine MPC-11 cells. The ED50 this effect is 0.3271 μg/ml in the presence of 7.5 ng/mL Activin A, corresponding to a specific activity is 3.06×10^3 units/mg.

  • Measured by its ability to neutralize Activin A inhibitory effect of murine MPC-11 cells. The ED50 for this effect is 0.3271 μg/ml in the presence of 7.5 ng/mL Activin A, corresponding to a specific activity is 3.06×103 units/mg.
种属

Human

表达系统

HEK293

标签

C-hFc

蛋白编号

NP_037541.1 (G30-W344)

基因 ID
蛋白结构
N-term
FST (G30-W344)
Accession # NP_037541.1
hFc
C-term
中文名
重组人激活素结合蛋白 (HEK293, Fc)
同用名
Follistatin; FS; Activin-binding protein; FST
氨基酸序列

GNCWLRQAKNGRCQVLYKTELSKEECCSTGRLSTSWTEEDVNDNTLFKWMIFNGGAPNCIPCKETCENVDCGPGKKCRMNKKNKPRCVCAPDCSNITWKGPVCGLDGKTYRNECALLKARCKEQPELEVQYQGRCKKTCRDVFCPGSSTCVVDQTNNAYCVTCNRICPEPASSEQYLCGNDGVTYSSACHLRKATCLLGRSIGLAYEGKCIKAKSCEDIQCTGGKKCLWDFKVGRGRCSLCDELCPDSKSDEPVCASDNATYASECAMKEAACSSGVLLEVKHSGSCNSISEDTEEEEEDEDQDYSFPISSILEW

分子量

Approximately 60.83 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

Follistatin/FST Protein, Human (HEK293, Fc) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Follistatin/FST Protein, Human (HEK293, Fc)
目录号:
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