FOXC1 蛋白, Human (C-His)

FOXC1 Protein, Human (C-His) is the recombinant human-derived FOXC1 protein, expressed by E. coli, with C-6*His tag.

FOXC1 是一种 DNA 结合转录因子，参与多种细胞和发育过程，包括眼睛、骨骼、心血管、肾脏和皮肤的发育。它可作为转录激活因子或抑制因子，结合靶基因启动子中的保守序列 5'-[G/C][A/T]AAA[T/C]AA[A/C]-3'，并在结合后促进 DNA 弯曲。FOXC1 还作为转录共激活因子，通过增强 GLI2 的 DNA 结合能力调节 Indian hedgehog (Ihh) 诱导的靶基因表达（By similarity），从而调控软骨内骨化。在乳腺癌细胞中，它作为转录共调节因子增强 GLI2 的 DNA 结合能力。FOXC1 通过结合 FOXO1 启动子区域的保守序列 5'-GTAAACAAA-3' 调节其表达，影响细胞存活和抗氧化应激能力。此外，它与 FOXC2 协同调节维持足细胞完整性的基因（By similarity），并通过 TGFB1 介导的信号通路抑制细胞周期 G1 期，从而抑制细胞生长。FOXC1 还参与上皮-间质转化 (EMT)，促进细胞增殖、迁移和侵袭，并在 CXCL12 诱导的内皮细胞迁移中调控 CXCR4 表达（By similarity）。此外，它对表皮角质形成细胞的终末分化至关重要，并在骨髓间充质祖细胞中正向调节 CXCL12 和干细胞因子表达，影响造血干细胞和祖细胞 (HSPC) 的微环境维持。在胚胎发育中，FOXC1 通过 VEGF 依赖性机制抑制血管和淋巴管生成，维持角膜透明性。部分研究表明，它可能具有肿瘤抑制功能。

Human

E. coli

C-6*His

Q12948 (M1-F553)

2296

Full Length

MQARYSVSSPNSLGVVPYLGGEQSYYRAAAAAAGGGYTAMPAPMSVYSHPAHAEQYPGGMARAYGPYTPQPQPKDMVKPPYSYIALITMAIQNAPDKKITLNGIYQFIMDRFPFYRDNKQGWQNSIRHNLSLNECFVKVPRDDKKPGKGSYWTLDPDSYNMFENGSFLRRRRRFKKKDAVKDKEEKDRLHLKEPPPPGRQPPPAPPEQADGNAPGPQPPPVRIQDIKTENGTCPSPPQPLSPAAALGSGSAAAVPKIESPDSSSSSLSSGSSPPGSLPSARPLSLDGADSAPPPPAPSAPPPHHSQGFSVDNIMTSLRGSPQSAAAELSSGLLASAAASSRAGIAPPLALGAYSPGQSSLYSSPCSQTSSAGSSGGGGGGAGAAGGAGGAGTYHCNLQAMSLYAAGERGGHLQGAPGGAGGSAVDDPLPDYSLPPVTSSSSSSLSHGGGGGGGGGGQEAGHHPAAHQGRLTSWYLNQAGGDLGHLASAAAAAAAAGYPGQQQNFHSVREMFESQRIGLNNSPVNGNSSCQMAFPSSQSLYRTSGAFVYDCSKF

Approximately 66 kDa, based on SDS-PAGE under reducing conditions.

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.