FPRP/PTGFRN 蛋白, Cynomolgus (HEK293, His)

Prostaglandin F2 receptor inhibitor (PTGFRN) is a type I transmembrane Ig superfamily cell adhesion molecule. PTGFRN is overexpressed in glioblastoma and promotes cell growth and radiation resistance through the PI3K-AKT signaling pathway. PTGFRN is involved in adipocyte maturation, muscle regeneration, tumor angiogenesis, metastasis, inhibition of follicle-stimulating hormone and luteinizing hormone secretion, and plasmodium infection. FPRP/PTGFRN Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived FPRP/PTGFRN protein, expressed by HEK293 , with C-His labeled tag. The total length of FPRP/PTGFRN Protein, Cynomolgus (HEK293, His) is 809 a.a., with molecular weight of 96-106 kDa.

前列腺素 F2 受体抑制剂 (PTGFRN) 是一种 I 型跨膜 Ig 超家族 细胞粘附分子 ，在包括胶质瘤在内的几种癌症中被证明是上调的。PTGFRN 在结直肠癌中表现为基因融合 (PTGFRN- notch2)，在小细胞肺癌中表现为点突变。它与四跨蛋白 (CD9 和 CD81)、整合素、Ezrin-Radixin-Moesin (ERM) 蛋白和 γ-分泌酶相互作用，调节细胞粘附和迁移。它还被发现参与脂肪细胞成熟、肌肉再生、肿瘤血管生成、转移、抑制促卵泡激素 (FSH) 和促黄体生成素 (LH) 分泌、疟原虫感染。PTGFRN 在胶质母细胞瘤中过表达，通过 PI3K-AKT 信号通路促进细胞生长和抗辐射。

Measured by its binding ability in a functional ELISA. When Recombinant Human FPRP/PTGFRN is immobilized at 5 µg/mL can bind Biotinylated Recombinant Human CD9. The ED₅₀ for this effect is ≤0.2867 μg/mL.

Cynomolgus

HEK293

C-His

H9ERH4 (R22-K830)

5738  [NCBI]

RVVRVPTGTLVRVVGTELVIPCNVSDYDGPSEQNFDWSFSSLGSSFVELASTWEVGFPAQLYQERLQRGEILLRRTANDAVELHIKNVQPSDQGHYKCSTPSTDATVQGNYEDTVQVKVLADSLHVGPSARPPPSLSLREGEPFELRCTAASASPLHTHLALLWELHRGPARRNVLALTHEGRFHPGLGYEQRYHSGDVRLDTVGSDAYRLSVSRALSADQGSYRCIVSEWIAEQGNWQEIQEKAVEVATVVIQPTVLRAAVPKNVSVAEGKELDLTCNITTDRADDVRPEVTWSFSRTPDSTLPGSRVLARLDRDSLVHSSPHIALSHVDARSYHLLVRDVSKGNSGYYFCHVSLWAPGHNRSWHKVAEAVSSPAGVGVTWLEPDYQVYLNASKVPGFADDPTELACRVVDTKSGEANVRFTVSWYYRMNRRSDDVVTSELLAVMDGDWTLKYGDRSKQRAQDGDFIFSKEHTDTFNFRIQRTTEEDRGNYYCVVSAWTKQRNNSWVKSKDVFSKPVNIFWALEDSVLVVKARQPKPFFAAGNTFEMTCKVSSKNIKSPRYSVLITAEKPVGDLSSPNETKYIISLDQDSVVKLENWTDASRVDGVVLEKVQEDEFRYRMYQTQVSDAGLYRCMVTAWSPVRGSLWREAATSLSNPIEIDFQTSGPIFNASVHSDTPSVIRGDLIKLFCIITVEGAALDPDDMAFDVSWFAVHSFGLDKAPVLLSSLDRKGIVTTSRRDWKSDLSLERVSVLEFLLQVHGSEDQDFGNYYCSVTPWVKSPTGSWQKEAEIHSKPVFITVKMDVLNAFK

Approximately 100-130 kDa due to the glycosylation

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.