Follistatin-like 1/FSTL1 蛋白, Mouse (HEK293, His)

Follistatin-like 1 (FSTL1) is a secreted glycoprotein that plays multiple roles in angiogenesis, immune regulation, and cell differentiation. It positively affects the development of the central nervous system, skeletal system, lungs and ureters. Follistatin-like 1/FSTL1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Follistatin-like 1/FSTL1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Follistatin-like 1/FSTL1 Protein, Mouse (HEK293, His) is 288 a.a., with molecular weight of 41-58 kDa.

卵泡抑素样 1 (FSTL1) 是一种分泌型糖蛋白，在多种生理过程中发挥多种作用，包括血管生成、免疫反应调节以及细胞增殖和分化。它积极参与中枢神经系统、骨骼系统、肺和输尿管的发育。FSTL1 通过 AKT 依赖性机制促进存活、迁移和分化为网络结构，从而对内皮细胞发挥影响，同时也支持心肌细胞的存活。该糖蛋白通过激活不同的细胞表面受体（包括 DIP2A、TLR4 和 BMP 受体）来启动信号级联反应。FSTL1 形成同二聚体并与 SCN10A、DIP2A（作为潜在的细胞表面受体）、BMP4 和 CD14 相互作用，后者相互作用促进 TLR4 介导的信号级联反应。这些相互作用凸显了 FSTL1 在协调各种细胞功能和信号通路方面的多方面性质。

Measured by its ability to inhibit the proliferation of OVCAR-3 cells. The ED₅₀ this effect is 15.17 ng/mL, corresponding to a specific activity is 6.592×10⁴ units/mg.

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  Measured by its ability to inhibit the proliferation of OVCAR-3 cells. The ED₅₀ for this effect is 15.17 ng/mL, corresponding to a specific activity is 6.592×10⁴ units/mg.

Mouse

HEK293

C-6*His

Q62356 (E19-I306)

14314  [NCBI]

EEEPRSKSKICANVFCGAGRECAVTEKGEPTCLCIEQCKPHKRPVCGSNGKTYLNHCELHRDACLTGSKIQVDYDGHCKEKKSASPSASPVVCYQANRDELRRRLIQWLEAEIIPDGWFSKGSNYSEILDKYFKSFDNGDSHLDSSEFLKFVEQNETAINITTYADQENNKLLRSLCVDALIELSDENADWKLSFQEFLKCLNPSFNPPEKKCALEDETYADGAETEVDCNRCVCSCGHWVCTAMTCDGKNQKGVQTHTEEEKTGYVQELQKHQGTAEKTKKVNTKEI

41-58 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.