G-CSF 蛋白, Human

G-CSF Protein, Human is a glycoprotein, acting to stimulate granulopoiesis, the innate immunity, and the differentiation of neural progenitor cells.

G-CSF 是一种糖蛋白，由免疫系统细胞、成纤维细胞和内皮细胞分泌，具有刺激粒细胞生成、先天免疫和神经祖细胞分化的作用。G-CSF 在 PI3K/Akt 通路磷酸化增加时向中枢神经元传递神经保护作用，并调节癌症中上皮细胞向间质细胞的转变^[1]。G-CSF 通过属于 I 类细胞因子受体超家族的特定同源受体 (G-CSFR) 发挥作用。G-CSF 是众所周知的造血细胞因子，可刺激骨髓祖细胞的增殖、分化和功能以及造血干细胞和祖细胞的动员^[2]。G-CSF 在动物模型中具有抑制动脉粥样硬化进展的潜力^[3]。

The ED₅₀ is <0.1 ng/mL as measured by M-NFS-60 cells, corresponding to a specific activity of >1.0 × 10⁷ units/mg.

• 
  Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED₅₀ for this effect is 10.17 pg/mL, corresponding to a specific activity is 9.83×10⁷ units/mg.

Human

E. coli

Tag Free

Q8N4W3 (T27-P200)/P09919-2 (T31-P204)

1440  [NCBI]

TPLGPASSLPQSFLLKCLEQVRKIQGDGAALQEKLCATYKLCHPEELVLLGHSLGIPWAPLSSCPSQALQLAGCLSQLHSGLFLYQGLLQALEGISPELGPTLDTLQLDVADFATTIWQQMEELGMAPALQPTQGAMPAFASAFQRRAGGVLVASHLQSFLEVSYRVLRHLAQP

Approximately 16-18 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 10 mM HAc-NaAc, 150 mM NaCl, 0.004% Tween 80, 5% Mannitol, pH 4.0 or 20 mM PB, 150 mM NaCl, pH 7.4 or 25 mM Tris, pH 8.0 or 10mM HAc-NaAc, 150 mM NaCl, pH 4.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Aliper AM, et al. A role for G-CSF and GM-CSF in nonmyeloid cancers. Cancer Med. 2014 Aug;3(4):737-46.  [Content Brief]

  [2]. Wright CR, et al. Granulocyte Colony-Stimulating Factor and Its Potential Application for Skeletal Muscle Repair and Regeneration. Mediators Inflamm. 2017;2017:7517350.  [Content Brief]

  [3]. Liu M, et al. The Effect of Granulocyte Colony-Stimulating Factor on the Progression of Atherosclerosis in Animal Models: A Meta-Analysis. Biomed Res Int. 2017;2017:6705363.  [Content Brief]