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  3. GADD45G/DDIT2 蛋白, Human (His)

GADD45G/DDIT2 蛋白, Human (His)

目录号: HY-P70218
产品使用指南

GADD45G/DDIT2 蛋白是通过 MTK1/MEKK4 MAPKKK 信号通路调节生长和凋亡的关键介质。浓度依赖性同二聚化增强了其生长抑制活性以及与 PCNA 的相互作用,表明其在 DNA 修复中的作用。GADD45G/DDIT2 蛋白, Human (His) 是重组的 GADD45G/DDIT2 蛋白,由 E. coli 表达,带有 N-6*His 标签。GADD45G/DDIT2 蛋白, Human (His) 全长 159 个氨基酸,分子量约为 ~21.0 kDa。

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描述

GADD45G/DDIT2 protein is a key mediator that regulates growth and apoptosis through the MTK1/MEKK4 MAPKKK signaling pathway. Concentration-dependent homodimerization enhances its growth inhibitory activity and interaction with PCNA, suggesting a role in DNA repair. GADD45G/DDIT2 Protein, Human (His) is the recombinant human-derived GADD45G/DDIT2 protein, expressed by E. coli , with N-6*His labeled tag. The total length of GADD45G/DDIT2 Protein, Human (His) is 159 a.a., with molecular weight of ~21.0 kDa.

研究背景

GADD45G/DDIT2 蛋白在生长和细胞凋亡的调节中发挥着关键作用,充当应激反应性 MTK1/MEKK4 MAPKKK 信号通路激活的介质。该蛋白质经历浓度依赖性同二聚化,这是其生长抑制活性的一个关键方面,并且这种同二聚化增强了其与 PCNA 的相互作用。GADD45G/DDIT2 进一步与 GADD45GIP1 相互作用,形成分子复合物,可能有助于调节细胞反应,特别是在应激条件下。此外,它与 PCNA 的相互作用表明在 DNA 修复过程中发挥作用,突显 GADD45G/DDIT2 在协调细胞对应激的反应以及影响控制生长和凋亡的重要途径方面的多方面功能。

种属

Human

表达系统

E. coli

标签

N-6*His

蛋白编号

O95257 (M1-E159)

基因 ID
蛋白结构
N-term
6*His
GADD45G (M1-E159)
Accession # O95257
C-term
同用名
rHuGrowth arrest and DNA damage-inducible protein GADD45 gamma/GADD45G, His; Growth Arrest and DNA Damage-Inducible Protein GADD45 Gamma; Cytokine-Responsive Protein CR6; DNA Damage-Inducible Transcript 2 Protein; DDIT-2; GADD45G; CR6; DDIT2
氨基酸序列

MTLEEVRGQDTVPESTARMQGAGKALHELLLSAQRQGCLTAGVYESAKVLNVDPDNVTFCVLAAGEEDEGDIALQIHFTLIQAFCCENDIDIVRVGDVQRLAAIVGAGEEAGAPGDLHCILISNPNEDAWKDPALEKLSLFCEESRSVNDWVPSITLPE

分子量

Approximately 21.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

GADD45G/DDIT2 Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
GADD45G/DDIT2 Protein, Human (His)
目录号:
HY-P70218
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