Gag-Pol polyprotein, HIV (560a.a, His)

The Gag-Pol polyprotein plays a central role in virion assembly, cooperating with Gag to bind to the plasma membrane, promote protein-protein interactions, recruit viral Env proteins, and package genomic RNA through direct interaction with the RNA packaging sequence (Psi). Gag-Pol polyprotein, HIV (560a.a, His) is the recombinant Virus-derived Gag-Pol polyprotein, expressed by E. coli , with N-6*His labeled tag. The total length of Gag-Pol polyprotein, HIV (560a.a, His) is 560 a.a., with molecular weight of ~66 KDa.

Gag-Pol 多蛋白在病毒体组装过程中发挥核心作用，与 Gag 多蛋白合作协调重要事件，例如与质膜结合、促进对球形颗粒形成至关重要的蛋白质-蛋白质相互作用、招募病毒包膜蛋白和包装基因组 RNA 通过与 RNA 包装序列 (Psi) 直接相互作用。这种多蛋白可能通过结合 5'-UTR 中的基因组 RNA 来调节其自身翻译，在低浓度下促进翻译和在较高浓度下包裹基因组 RNA 抑制翻译方面表现出双重作用。多部分膜结合信号，包括肉豆蔻酰化的 N 末端，将多蛋白靶向质膜。此外，多蛋白内的基质蛋白涉及预整合复合物，并与Vpu介导的宿主细胞释放相关。Gag-Pol 与 RNA 结合的能力进一步强调了其在病毒粒子组装的复杂过程中的多方面功能。

Data is not available.

Virus

E. coli

N-6*His

P04585 (P588-L1147)

155348  [NCBI]

PISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKDSTKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKKKKSVTVLDVGDAYFSVPLDEDFRKYTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQSSMTKILEPFRKQNPDIVIYQYMDDLYVGSDLEIGQHRTKIEELRQHLLRWGLTTPDKKHQKEPPFLWMGYELHPDKWTVQPIVLPEKDSWTVNDIQKLVGKLNWASQIYPGIKVRQLCKLLRGTKALTEVIPLTEEAELELAENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMRGAHTNDVKQLTEAVQKITTESIVIWGKTPKFKLPIQKETWETWWTEYWQATWIPEWEFVNTPPLVKLWYQLEKEPIVGAETFYVDGAANRETKLGKAGYVTNRGRQKVVTLTDTTNQKTELQAIYLALQDSGLEVNIVTDSQYALGIIQAQPDQSESELVNQIIEQLIKKEKVYLAWVPAHKGIGGNEQVDKLVSAGIRKVL

Approximately 66-68 kDa.

• 
  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.