Galectin-3/LGALS3 蛋白, Human

Galectin-3/LGALS3 Protein is a type of galactoside-binding lectin that has a high binding affinity for carbohydrates with β-galactoside linkages. Galectin-3/LGALS3 Protein interacts with glycosylated proteins to mediate intercellular interactions and adhesion to the extracellular matrix. Galectin-3/LGALS3 Protein plays various roles, including regulating signaling pathways such as Wnt/β-catenin, affecting cell proliferation and survival, modulating immune functions, and influencing tumor progression. Galectin-3/LGALS3 Protein, Human is a recombinant galectin-3/LGALS3 protein expressed in E. coli^[1][2].

Galectin-3/LGALS3 Protein 蛋白广泛分布在大多数组织部位，并在特定组织中广泛表达，具有细胞外、膜结合、细胞质和核定位^[1]。
Galectin-3/LGALS3 Protein 的优选配体是 N-乙酰乳糖胺，也是唯一一种包含保守 N 结构域和单个 CRD 结构域的半乳糖凝集素，其中 N 结构域允许不与碳水化合物靶标结合的蛋白形成多聚体复合物^[1]。
Galectin-3/LGALS3 Protein 具有多种细胞功能，包括调控环境敏感的细胞粘附或裂开，调节网格蛋白非依赖性内吞作用，通过间隔膜结合信号复合物来调节细胞内信号转导，调节 Wnt/β-catenin 信号转导，影响 TTF-1 和 STAT 转录因子活性，通过对剪接体功能的影响调节 mRNA 成熟，修复 DNA 损伤，诱导晚期 G1 细胞周期停滞，促进细胞增殖和通过 Bcl2 的抗凋亡作用促进细胞存活^[1]。
Galectin-3/LGALS3 Protein 参与免疫功能。它通过调理细胞碎片、促进呼吸爆发酶的产生、作为 MerTK 特异性的吞噬信号和作为中枢神经系统的警告信号来促进先天免疫；此外，还可以通过调节 T 细胞活化来促进获得性免疫^[2]。
Galectin-3/LGALS3 Protein 与肿瘤、心血管疾病、急性肾损伤等病理过程密切相关^[3][4][5]。

Galectin-3/LGALS3 Protein 在 MI D1 巨噬细胞中的高度表达可以促进中性粒细胞脱颗粒从而调节心肌梗死^[3]。
Galectin-3/LGALS3 Protein 在非小细胞肺癌中的过表达可能与肺癌的发生发展有关^[4]。
Galectin-3/LGALS3 Protein 在 LGALS3 基因敲低的小鼠远曲小管 (DCT) 细胞中的表达缺乏可以促进 AGE 介导的细胞凋亡^[5]。
Galectin-3/LGALS3 Protein 在 LGALS3 基因沉默的转移性黑色素瘤细胞中的表达缺乏可以抑制促肿瘤基因 autotaxin (ENPP2) 和转录因子 NFAT1 的表达^[6]。

Galectin-3/LGALS3 Protein 在 LGALS3 突变 (LGALS3 ^-/-) 小鼠中的表达缺乏会导致小鼠在进食、饮水和运动方面的昼夜节律模式更加多样化^[1]。
Galectin-3/LGALS3 Protein 在横纹肌溶解症引起的急性肾损伤 (RIAKI) 小鼠中表达水平升高，从而可以保护远端肾单位免受 AGE 介导的损伤^[5]。
Galectin-3/LGALS3 Protein 在 LGALS3 基因沉默的黑色素瘤小鼠中缺乏表达，从而通过抑制 NFAT1 和 autotaxin 的表达来促进黑色素瘤的生长转移^[6]。

Measured by the ability of the immobilized protein to support the adhesion of MOLT-4 human acute lymphoblastic leukemia cells. The ED₅₀ for this effect is 1-3 μg/mL.

• 
  Measured by its ability to induce IL-8 secretion in HT1080 human fibrosarcoma cells transfected with human OX40/TNFRSF4. The ED₅₀ for this effect is 3.527ng/mL, corresponding to a specific activity is 2.836×10⁵ U/mg.

Human

E. coli

Tag Free

P17931 (A2-I250)

3958  [NCBI]

Full Length of Mature Protein

ADNFSLHDALSGSGNPNPQGWPGAWGNQPAGAGGYPGASYPGAYPGQAPPGAYPGQAPPGAYPGAPGAYPGAPAPGVYPGPPSGPGAYPSSGQPSATGAYPATGPYGAPAGPLIVPYNLPLPGGVVPRMLITILGTVKPNANRIALDFQRGNDVAFHFNPRFNENNRRVIVCNTKLDNNWGREERQSVFPFESGKPFKIQVLVEPDHFKVAVNDAHLLQYNHRVKKLNEISKLGISGDIDLTSASYTMI

Approximately 29 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

1.Lyophilized from a 0.2 μm filtered solution of 50 mM HEPES, 5% Sucrose, 5% Mannitol, 0.06% Tween 80, pH 7.5.
2.Lyophilized from a 0.2 μm filtered solution of PBS, 8% trehalose, pH 7.4.
3.Lyophilized from a 0.2 μm filtered solution of 50 mM HEPES, 150 mMNaCl, 5% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Chaudoin TR, et al. Mice lacking galectin-3 (Lgals3) function have decreased home cage movement. BMC Neurosci. 2018 May 2;19(1):27.  [Content Brief]

  [2]. Demetriou M, et al. Negative regulation of T-cell activation and autoimmunity by Mgat5 N-glycosylation. Nature. 2001 Feb 8;409(6821):733-9.  [Content Brief]

  [3]. Chalise U, et al. Macrophages secrete murinoglobulin-1 and galectin-3 to regulate neutrophil degranulation after myocardial infarction. Mol Omics. 2022 Mar 28;18(3):186-195.  [Content Brief]

  [4]. Yoshimura A, et al. Increased expression of the LGALS3 (galectin 3) gene in human non-small-cell lung cancer. Genes Chromosomes Cancer. 2003 Jun;37(2):159-64.  [Content Brief]

  [5]. Kulow VA, et al. Galectin-3 protects distal convoluted tubules in rhabdomyolysis-induced kidney injury. Pflugers Arch. 2024 Jul 23.  [Content Brief]

  [6]. Braeuer RR, et al. Galectin-3 contributes to melanoma growth and metastasis via regulation of NFAT1 and autotaxin. Cancer Res. 2012 Nov 15;72(22):5757-66.  [Content Brief]