GALNT2 蛋白, Human (HEK293, His)

The GALNT2 protein catalyzes the initial steps in O-linked oligosaccharide biosynthesis by transferring N-acetyl-D-galactosamine to a protein receptor. GALNT2 Protein, Human (HEK293, His) is the recombinant human-derived GALNT2 protein, expressed by HEK293 , with N-His labeled tag. The total length of GALNT2 Protein, Human (HEK293, His) is 520 a.a., with molecular weight of 63 KDa, respectively.

GALNT2 蛋白是 O-连接寡糖生物合成中的关键酶，通过将 N-乙酰基-D-半乳糖胺残基转移到蛋白受体上的丝氨酸或苏氨酸残基来启动该过程。这种酶活性表现出广泛的底物谱，包括 EA2、Muc5AC、Muc1a 和 Muc1b 等肽。GALNT2 参与关键蛋白质的 O 连接糖基化，包括免疫球蛋白 A1 (IgA1) 铰链区、APOC-III、ANGPTL3 和 PLTP。此外，它还在高密度脂蛋白胆固醇 (HDL-C) 代谢的调节中发挥作用，有助于控制脂质稳态的复杂过程。

Measured by its ability to transfer GalNAc from UDP-GalNAc to EA2 that incubate at 37°C for 20 min. The specific activity is 379.51 pmol/min/μg.

Human

HEK293

N-10*His

Q10471-1/NP_004472.1 (K52-Q571)

2590  [NCBI]

KKKDLHHSNGEEKAQSMETLPPGKVRWPDFNQEAYVGGTMVRSGQDPYARNKFNQVESDKLRMDRAIPDTRHDQCQRKQWRVDLPATSVVITFHNEARSALLRTVVSVLKKSPPHLIKEIILVDDYSNDPEDGALLGKIEKVRVLRNDRREGLMRSRVRGADAAQAKVLTFLDSHCECNEHWLEPLLERVAEDRTRVVSPIIDVINMDNFQYVGASADLKGGFDWNLVFKWDYMTPEQRRSRQGNPVAPIKTPMIAGGLFVMDKFYFEELGKYDMMMDVWGGENLEISFRVWQCGGSLEIIPCSRVGHVFRKQHPYTFPGGSGTVFARNTRRAAEVWMDEYKNFYYAAVPSARNVPYGNIQSRLELRKKLSCKPFKWYLENVYPELRVPDHQDIAFGALQQGTNCLDTLGHFADGVVGVYECHNAGGNQEWALTKEKSVKHMDLCLTVVDRAPGSLIKLQGCRENDSRQKWEQIEGNSKLRHVGSNLCLDSRTAKSGGLSVEVCGPALSQQWKFTLNLQQ

Approximately 63 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 25 mM Tris, 150 mM NaCl, pH 7.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.