1. 重组蛋白
  2. Enzymes & Regulators
  3. Lyases (EC 4)
  4. Gamma-enolase/Enolase 2 蛋白, Human (His)

Gamma-enolase/Enolase 2 蛋白, Human (His)

目录号: HY-P70271
COA 产品使用指南

γ-烯醇化酶/烯醇化酶 2 蛋白具有神经营养和神经保护特性,与新皮质神经元结合并通过钙依赖性机制促进细胞存活。这表明它在支持中枢神经系统内神经元活力方面具有潜在作用。Gamma-enolase/Enolase 2 蛋白, Human (His) 是重组的 γ-烯醇化酶/烯醇化酶 2 蛋白,由 E. coli 表达,带有 N-6*His 标签。Gamma-enolase/Enolase 2 蛋白, Human (His) 全长 434 个氨基酸,分子量约为 ~60 kDa。

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规格 价格 是否有货 数量
10 μg ¥1600 In-stock
50 μg ¥4500 In-stock
100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

Gamma-enolase/Enolase 2 protein exhibits neurotrophic and neuroprotective properties, binding to neocortical neurons and promoting cell survival through a calcium-dependent mechanism. This suggests its potential role in supporting neuron viability within the central nervous system. Gamma-enolase/Enolase 2 Protein, Human (His) is the recombinant human-derived Gamma-enolase/Enolase 2 protein, expressed by E. coli , with N-6*His labeled tag. The total length of Gamma-enolase/Enolase 2 Protein, Human (His) is 434 a.a., with molecular weight of ~60 kDa.

研究背景

γ-烯醇化酶/烯醇化酶 2 蛋白在多种中枢神经系统 (CNS) 神经元中表现出神经营养和神经保护特性。通过钙依赖性机制,它与培养的新皮质神经元结合,有效促进细胞存活。这些发现表明,γ-烯醇化酶/烯醇化酶 2 在支持中枢神经系统内神经元的活力方面具有潜在作用。

生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

种属

Human

表达系统

E. coli

标签

N-6*His

蛋白编号

P09104 (M1-L434)

基因 ID
蛋白结构
N-term
6*His
ENO2 (M1-L434)
Accession # P09104
C-term
同用名
rHuGamma-enolase/ENO2, His; Gamma-enolase; 2-phospho-D-glycerate hydro-lyase; Enolase 2; Neural enolase; Neuron-specific enolase; NSE; ENO2
氨基酸序列

MSIEKIWAREILDSRGNPTVEVDLYTAKGLFRAAVPSGASTGIYEALELRDGDKQRYLGKGVLKAVDHINSTIAPALISSGLSVVEQEKLDNLMLELDGTENKSKFGANAILGVSLAVCKAGAAERELPLYRHIAQLAGNSDLILPVPAFNVINGGSHAGNKLAMQEFMILPVGAESFRDAMRLGAEVYHTLKGVIKDKYGKDATNVGDEGGFAPNILENSEALELVKEAIDKAGYTEKIVIGMDVAASEFYRDGKYDLDFKSPTDPSRYITGDQLGALYQDFVRDYPVVSIEDPFDQDDWAAWSKFTANVGIQIVGDDLTVTNPKRIERAVEEKACNCLLLKVNQIGSVTEAIQACKLAQENGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLMRIEEELGDEARFAGHNFRNPSVL

分子量

Approximately 60 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 100 mM KCl, 5 mM MgSO4, pH 7.5.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Gamma-enolase/Enolase 2 Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Gamma-enolase/Enolase 2 Protein, Human (His)
目录号:
HY-P70271
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