1. 重组蛋白
  2. Viral Proteins
  3. Bacterial/Fungal Proteins
  4. Gamma-hemolysin component C 蛋白, S. aureus (P.pastoris, His)

Gamma-hemolysin component C 蛋白, S. aureus (P.pastoris, His)

目录号: HY-P71835
产品使用指南

γ-溶血素成分 C (HLgC) 充当毒素,形成具有溶血和白细胞毒性活性的细胞膜孔。HlgC 的毒性需要与 S 类和 F 类成分顺序结合并协同缔合,形成异寡聚复合物。Gamma-hemolysin component C 蛋白, S. aureus (P.pastoris, His) 是重组的 γ-溶血素成分 C 蛋白,由 P. pastoris 表达,带有 N-His 标签。Gamma-hemolysin component C 蛋白, S. aureus (P.pastoris, His) 全长 286 个氨基酸,分子量约为 ~34.6 kDa。

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描述

Gamma-hemolysin component C (HLgC) acts as a toxin, forming cell membrane pores with hemolytic and leukotoxic activities. The toxicity of HlgC requires sequential binding and cooperative association with S- and F-class components to form heterooligomeric complexes. Gamma-hemolysin component C Protein, S. aureus (P.pastoris, His) is the recombinant Staphylococcus aureus-derived Gamma-hemolysin component C protein, expressed by P. pastoris , with N-His labeled tag. The total length of Gamma-hemolysin component C Protein, S. aureus (P.pastoris, His) is 286 a.a., with molecular weight of ~34.6 kDa.

研究背景

γ-溶血素成分 C (HLgC) 是一种毒素,其作用机制涉及细胞膜上孔的形成,从而导致溶血和白细胞毒性活性。HlgC 的毒性需要 S 类和 F 类成分的顺序结合和协同缔合,从而形成异寡聚复合物。特别是,属于S类的HlgC与F类成分HlgB结合,形成CB毒素。这种协同相互作用强调了 HlgC 对靶细胞发挥毒性作用的复杂分子过程。

种属

Staphylococcus aureus

表达系统

P. pastoris

标签

N-His

蛋白编号

Q7A3S2 (30A-315N)

基因 ID

/

蛋白结构
N-term
His
hlgC (30A-315N)
Accession # Q7A3S2
C-term
同用名
hlgC; SA2208Gamma-hemolysin component C
氨基酸序列

ANDTEDIGKGSDIEIIKRTEDKTSNKWGVTQNIQFDFVKDKKYNKDALILKMQGFISSRTTYYNYKKTNHVKAMRWPFQYNIGLKTNDKYVSLINYLPKNKIESTNVSQTLGYNIGGNFQSAPSLGGNGSFNYSKSISYTQQNYVSEVEQQNSKSVLWGVKANSFATESGQKSAFDSDLFVGYKPHSKDPRDYFVPDSELPPLVQSGFNPSFIATVSHEKGSSDTSEFEITYGRNMDVTHAIKRSTHYGNSYLDGHRVHNAFVNRNYTVKYEVNWKTHEIKVKGQN

分子量

Approximately 34.6 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

Gamma-hemolysin component C Protein, S. aureus (P.pastoris, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Gamma-hemolysin component C Protein, S. aureus (P.pastoris, His)
目录号:
HY-P71835
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