GAP43 蛋白, Human (HEK293, His)

The GAP43 protein plays a central role in nerve growth and is found primarily in the active "growth cones" at the tips of axons. It induces axonal and dendritic filopodia, which are critical for neurodevelopmental dynamics. GAP43 Protein, Human (HEK293, His) is the recombinant human-derived GAP43 protein, expressed by HEK293 , with C-His labeled tag. The total length of GAP43 Protein, Human (HEK293, His) is 238 a.a., with molecular weight of ~47 kDa.

GAP43 蛋白与神经生长密切相关，是在伸长轴突尖端形成的活动“生长锥”内的主要成分。它的关键作用延伸到轴突和树突丝状伪足的诱导，有助于对神经发育至关重要的动态过程。在分子背景下，GAP43 在包含 FGFR4、NCAM1、CDH2、PLCG1、FRS2、SRC、SHC1 和 CTTN 的复合物中被鉴定，表明其参与多种信号传导途径。此外，GAP43 表现出与钙调蛋白的相互作用，由其 IQ 结构域介导，在不存在 Ca(2+) 的情况下与钙调蛋白的结合亲和力比存在 Ca(2+) 时更高。这些复杂的分子关联强调了 GAP43 在神经生长和信号级联中的多方面参与，揭示了其在协调轴突动力学和丝状伪足形成中的重要性。

Human

HEK293

C-His

P17677 (M1-A238)

2596  [NCBI]

Approximately 47 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.