1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. Gastric lipase 蛋白, Human (HEK293, His)

Gastric lipase 蛋白, Human (HEK293, His)

目录号: HY-P70262
COA 产品使用指南

胃脂肪酶是消化过程中的关键酶,在三酰甘油水解成游离脂肪酸、二酰甘油、单酰甘油和甘油中起关键作用。这种酶活性对胃环境中膳食脂肪的分解有显着贡献。Gastric lipase 蛋白, Human (HEK293, His) 是重组的 胃脂肪酶蛋白,由 HEK293 表达,带有 C-6*His 标签。Gastric lipase 蛋白, Human (HEK293, His) 全长 379 个氨基酸,分子量约为 ~53.85 kDa。

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规格 价格 是否有货 数量
10 μg ¥1540 In-stock
50 μg   询价  
100 μg   询价  

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描述

Gastric lipase is a key enzyme in the digestive process and plays a key role in the hydrolysis of triacylglycerols into free fatty acids, diacylglycerols, monoacylglycerols, and glycerol. This enzyme activity contributes significantly to the breakdown of dietary fat in the gastric environment. Gastric lipase Protein, Human (HEK293, His) is the recombinant human-derived Gastric lipase protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Gastric lipase Protein, Human (HEK293, His) is 379 a.a., with molecular weight of ~53.85 kDa.

研究背景

胃脂肪酶是一种催化三酰甘油水解,将其分解成游离脂肪酸、二酰甘油、单酰甘油和甘油的酶。这种水解活性在胃中膳食脂肪的消化中起着至关重要的作用。胃脂肪酶优先水解三酰甘油分子的 sn-3 位。通过选择性地靶向三酰甘油内的特定键,胃脂肪酶有助于生成更小的脂质分子,这些分子可以在消化过程中进一步加工和吸收。它必须强调酶的底物特异性及其在脂肪消化的初始步骤中的重要性。

生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

AAI12273.1 (L20-K398)

基因 ID
蛋白结构
N-term
LIPF (L20-K398)
Accession # AAI12273.1
6*His
C-term
同用名
rHuGastric triacylglycerol lipase/LIPF, His ; Gastric Triacylglycerol Lipase; GL; Gastric Lipase; LIPF
氨基酸序列

LFGKLHPGSPEVTMNISQMITYWGYPNEEYEVVTEDGYILEVNRIPYGKKNSGNTGQRPVVFLQHGLLASATNWISNLPNNSLAFILADAGYDVWLGNSRGNTWARRNLYYSPDSVEFWAFSFDEMAKYDLPATIDFIVKKTGQKQLHYVGHSQGTTIGFIAFSTNPSLAKRIKTFYALAPVATVKYTKSLINKLRFVPQSLFKFIFGDKIFYPHNFFDQFLATEVCSREMLNLLCSNALFIICGFDSKNFNTSRLDVYLSHNPAGTSVQNMFHWTQAVKSGKFQAYDWGSPVQNRMHYDQSQPPYYNVTAMNVPIAVWNGGKDLLADPQDVGLLLPKLPNLIYHKEIPFYNHLDFIWAMDAPQEVYNDIVSMISEDKK

分子量

Approximately 53.85 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

溶液.

组分

Supplied as a 0.2 μm filtered solution of 25 mM Tris-HCl, 100 mM Glycine, 10% Glycerol, pH 7.3.

内毒素含量

<1 EU/μg, determined by LAL method.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料

Gastric lipase Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Gastric lipase Protein, Human (HEK293, His)
目录号:
HY-P70262
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