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  4. GCAP1 蛋白, Human (sf9)

GCAP1 蛋白, Human (sf9)

目录号: HY-P76944
产品使用指南

GCAP1 蛋白是视觉系统中的关键调节因子，根据细胞内钙浓度调节视网膜鸟苷酸环化酶活性。在低钙条件下，GCAP1 刺激视网膜鸟苷酸环化酶，使视杆光感受器恢复暗态。钙浓度升高会抑制鸟苷酸环化酶活性，这是视杆细胞光感受器光转导的一个重要方面。此外，GCAP1 可能有助于强光下视锥细胞感光器的光响应和恢复。该蛋白的同源二聚体结构增强了其在视觉信号通路中的调节能力。GCAP1 蛋白, Human (sf9) 是重组的 GCAP1 蛋白，由 Sf9 insect cells 表达，不带标签和 with residual Gly-Pro in N-terminal (not related to functional changes) 突变。GCAP1 蛋白, Human (sf9) 全长 201 个氨基酸，分子量约为 ~20 kDa。

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描述

GCAP1 Proteinas, a pivotal regulator in the visual system, modulates retinal guanylyl cyclase activity based on intracellular calcium concentrations. Under low calcium, GCAP1 stimulates retinal guanylyl cyclase for dark state recovery in rod photoreceptors. Elevated calcium inhibits guanylyl cyclase activity, a crucial aspect of rod photoreceptor phototransduction. Additionally, GCAP1 may contribute to light response and recovery in cone photoreceptors under bright light. The protein's homodimeric structure enhances its regulatory capabilities in the visual signaling pathway. GCAP1 Protein, Human (sf9) is the recombinant human-derived GCAP1 protein, expressed by Sf9 insect cells , with tag free. and with residual Gly-Pro in N-terminal (not related to functional changes) mutation. The total length of GCAP1 Protein, Human (sf9) is 201 a.a., with molecular weight of ~20 kDa.
研究背景

GCAP1 蛋白是视觉系统中的关键调节因子，在根据细胞内钙浓度调节视网膜鸟苷酸环化酶活性方面发挥双重作用。在低游离钙离子条件下，GCAP1 刺激视网膜鸟苷酸环化酶，促进视杆光感受器在光照后恢复黑暗状态。相反，当游离钙离子浓度升高时，GCAP1 会抑制鸟苷酸环化酶活性。这种钙敏感控制机制代表了视杆光感受器中光转导过程的一个关键方面。此外，GCAP1 可能在强光条件下锥体感光细胞的光响应和恢复中发挥作用。该蛋白质作为同二聚体发挥作用，进一步增强其在视觉信号通路中的调节能力。
种属

Human
表达系统

Sf9 insect cells
标签

Tag Free
蛋白编号

P43080 (N-G&P, M1-G201)
基因 ID

118142757  [NCBI]
蛋白结构
N-term
Gly-Pro
GCAP1 (M1-G201)
Accession # P43080
C-term
同用名
Guanylyl cyclase-activating protein 1; Guanylate cyclase activator 1A; C6orf131; GCAP; GUCA1; GUCA1A
分子量

Approximately 20 kDa
纯度

Greater than 85% as determined by reducing SDS-PAGE
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

GCAP1 Protein, Human (sf9) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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