GFP 蛋白, Aequorea victoria (His)

The GFP protein acts as an energy transfer receptor and is actively involved in the transduction of blue chemiluminescence produced by the protein aequorin. Its main function is to convert this chemical energy into green fluorescence through an efficient energy transfer process. GFP Protein, Aequorea victoria (His) is the recombinant GFP protein, expressed by E. coli , with N-6*His labeled tag. The total length of GFP Protein, Aequorea victoria (His) is 238 a.a., with molecular weight of 27-31 kDa.

绿色荧光蛋白（GFP）作为能量转移受体，通过能量转移将蛋白质水母发光蛋白发出的蓝色化学发光转化为绿色荧光。GFP 独特的体内荧光能力取决于从 Ca(2+) 激活的发光蛋白水母发光蛋白接收能量。这一非凡的特性使 GFP 成为分子和细胞生物学中的宝贵工具，使活体中特定蛋白质和细胞结构的可视化成为可能。使用 GFP 作为荧光标记彻底改变了生物学研究，促进了动态细胞过程的非侵入性实时成像（参考：提供的信息）。

Others

E. coli

N-6*His

P42212 (M1-K238)

/

MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK

27-31 kDa

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  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized a 0.2 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0 or 50 mM Tris, 300 mM NaCl, 5% trehalose, 5% mannitol and 0.01% Tween 80, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 1 year, protect from light. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.