1. 重组蛋白
  2. Enzymes & Regulators
  3. Lyases (EC 4)
  4. GGCT 蛋白, Human (His)

GGCT 蛋白, Human (His)

目录号: HY-P70211
COA 产品使用指南

GGCT 蛋白是硫酸盐同化的关键参与者,催化无机硫酸盐和 ATP 转化为腺苷-5'-磷酸硫酸盐 (APS) 的第一步。这种活性对于含硫氨基酸的生物合成至关重要,并且对于细胞硫代谢至关重要。GGCT 蛋白, Human (His) 是重组的 GGCT 蛋白,由 E. coli 表达,带有 N-6*His 标签。GGCT 蛋白, Human (His) 全长 188 个氨基酸,分子量约为 ~23.0 kDa。

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规格 价格 是否有货 数量
10 μg ¥1550 In-stock
50 μg ¥4300 In-stock
100 μg   询价  

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描述

The GGCT protein is a key player in sulfate assimilation, catalyzing the initial step in the conversion of inorganic sulfate and ATP to adenosine-5'-phosphate sulfate (APS). This activity is essential for the biosynthesis of sulfur-containing amino acids and is essential for cellular sulfur metabolism. GGCT Protein, Human (His) is the recombinant human-derived GGCT protein, expressed by E. coli , with N-6*His labeled tag. The total length of GGCT Protein, Human (His) is 188 a.a., with molecular weight of ~23.0 kDa.

研究背景

ATP 硫化酶/MET3 蛋白通过催化将无机硫酸盐和 ATP 转化为腺苷-5'-磷酸硫酸盐 (APS) 的初始细胞内反应,在硫酸盐同化中发挥关键作用。这种酶活性是含硫氨基酸生物合成途径中的关键步骤,对细胞硫代谢有显着贡献。通过促进硫酸盐的活化,ATP 硫化酶/MET3 在确保关键生物分子合成所需的硫的可用性方面发挥着重要作用,强调了其在维持细胞含硫氨基酸库和支持基本细胞过程中的重要性。

生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

种属

Human

表达系统

E. coli

标签

N-6*His

蛋白编号

O75223 (M1-L188)

基因 ID
蛋白结构
N-term
6*His
GGCT (M1-L188)
Accession # O75223
C-term
同用名
rHuGamma-glutamylcyclotransferase/GGCT, His; Gamma-Glutamylcyclotransferase; Cytochrome C-Releasing Factor 21; GGCT; C7orf24; CRF21
氨基酸序列

MANSGCKDVTGPDEESFLYFAYGSNLLTERIHLRNPSAAFFCVARLQDFKLDFGNSQGKTSQTWHGGIATIFQSPGDEVWGVVWKMNKSNLNSLDEQEGVKSGMYVVIEVKVATQEGKEITCRSYLMTNYESAPPSPQYKKIICMGAKENGLPLEYQEKLKAIEPNDYTGKVSEEIEDIIKKGETQTL

分子量

Approximately 23.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

溶液.

组分

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, pH 8.0.

内毒素含量

<1 EU/μg, determined by LAL method.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料

GGCT Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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GGCT Protein, Human (His)
目录号:
HY-P70211
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