1. 重组蛋白
  2. Cytokines and Growth Factors Immune Checkpoint Proteins CD Antigens Biotinylated Proteins
  3. TNF Superfamily Stimulatory immune checkpoint molecules Macrophage CD Proteins Monocyte CD Proteins
  4. TNF Receptor Superfamily GITR/CD357
  5. GITR
  6. GITR 蛋白, Human (Biotinylated, HEK293, His-Avi)

GITR 蛋白, Human (Biotinylated, HEK293, His-Avi)

目录号: HY-P78137
产品使用指南

GITR (TNFRSF18) 是 TNFR 超家族的成员。GITR 促进 T 细胞活化和增殖,增加对肿瘤和病毒感染的抵抗力,并加剧自身免疫性疾病和炎症过程。GITR 蛋白, Human (Biotinylated, HEK293, His-Avi) 是一种由 HEK293 细胞表达的生物素化的重组蛋白,其 C 末端带有一个 Avi 标签和一个 His 标签,由 136 (Q26-E161) 个氨基酸组成。

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重组蛋白定制服务

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     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

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100 μg 询问价格 & 货期

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描述

GITR (TNFRSF18) is a member of the TNFR superfamily. GITR promotes T cell activation and proliferation and increases resistance to tumors and viral infections, and exacerbates autoimmune diseases and inflammation processes[1]. GITR Protein, Human (Biotinylated, HEK293, His-Avi) is a biotinylated recombinant protein with a C-terminal Avi label and a C-terminal His label, It consists of 136 amino acids (Q26-E161) and is produced in HEK293 cells.

研究背景

GITR is expressed on regulatory T cells (Tregs) and some activated immune cells, including effector T lymphocytes, nature killer (NK) cells, and neutrophils[1].
The amino acid sequence of human GITR protein has low homology for mouse GITR protein.
GITR does not have any enzymatic activity and signaling is propagated via recruiting TRAF-family members, specifically TRAF1, TRAF2 and TRAF5, to the GITR-signaling complex. The signaling is then mediated through NF-kB and MAPK pathways. GITR does not have any enzymatic activity and signaling is propagated via recruiting TRAF-family members, specifically TRAF1, TRAF2 and TRAF5, to the GITR-signaling complex. The signaling is then mediated through NF-kB and MAPK pathways, protecting T cells from TCR activation-induced cell death[2].
GITR (Glucocorticoid-induced TNFR-related protein, also known as TNFRSF18) is a type I transmembrane protein. GITR stimulates the proliferation of effector T-lymphocytes and partially reverses the immunosuppressive function of CD4+CD25+ Tregs[1]. GITR is activated by its ligand GITRL (TNFSF18). GITR induces NOS in murine macrophage in a time and dose-dependent manner[3]. GITR inhibits Multiple Myeloma (MM) cell proliferation in vitro and in vivo and induces apoptosis[4].

生物活性

Immobilized Human GITR Ligand Trimer hFc at 5 μg/mL (100μL/Well) on the plate. Dose response curve for Biotinylated Human GITR His with the EC50 of 0.14 μg/mL determined by ELISA.

种属

Human

表达系统

HEK293

标签

C-Avi;C-His

蛋白编号

Q9Y5U5 (Q26-E161)

基因 ID

8784  [NCBI]

中文名
重组人 GITR 蛋白 (Biotinylated, HEK293, His-Avi)
同用名
CD357; GITR; GITR-D; TNFRSF18; AITR
分子量

26-28 kDa

纯度

Greater than 95% as determined by Tris-Bis PAGE.

性状

Solution.

组分

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

N/A.

保存条件 & 期限

Stored at -80°C. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料
参考文献
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
GITR Protein, Human (Biotinylated, HEK293, His-Avi)
目录号:
HY-P78137
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