Glucosamine (N-acetyl)-6-Sulfatase/GNS 蛋白, Human (HEK293, His)

Glucosamine (N-acetyl)-6-Sulfatase/GNS Protein is a lysosomal enzyme present in all cells. It is involved in the catabolism of heparin, heparin sulfate and keratin sulfate. Deficiency of this enzyme leads to inadequate substrate accumulation and lysosomal storage impairment IIID mucopolysaccharidosis. Glucosamine (N-acetyl) -6-Sulfatase/GNS Protein, Human (HEK293, His) is the recombinant human-derived Glucosamine, expressed by HEK293 , with C-6*His labeled tag. The total length of Glucosamine (N-acetyl) -6-Sulfatase/GNS Protein, Human (HEK293, His) is 516 a.a., with molecular weight of ~90 kDa.

硫酸酯酶可催化从各种底物水解 O-和 N-硫酸盐酯。大多数硫酸酯酶存在于溶酶体中。用于从糖胺聚糖 (GAGs)、糖肽和糖脂 (GLs) 中去除硫酸盐。N-acetylglucosamine-6-sulfatase 是一种存在于所有细胞中的溶酶体酶。它参与肝素、硫酸肝素和硫酸角蛋白的分解代谢。这种酶的缺乏会导致底物积累不足和溶酶体储存障碍 IIID 型粘多糖病。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

HEK293

C-6*His

P15586 (V37-L552)

2799  [NCBI]

VFGVAAGTRRPNVVLLLTDDQDEVLGGMTPLKKTKALIGEMGMTFSSAYVPSALCCPSRASILTGKYPHNHHVVNNTLEGNCSSKSWQKIQEPNTFPAILRSMCGYQTFFAGKYLNEYGAPDAGGLEHVPLGWSYWYALEKNSKYYNYTLSINGKARKHGENYSVDYLTDVLANVSLDFLDYKSNFEPFFMMIATPAPHSPWTAAPQYQKAFQNVFAPRNKNFNIHGTNKHWLIRQAKTPMTNSSIQFLDNAFRKRWQTLLSVDDLVEKLVKRLEFTGELNNTYIFYTSDNGYHTGQFSLPIDKRQLYEFDIKVPLLVRGPGIKPNQTSKMLVANIDLGPTILDIAGYDLNKTQMDGMSLLPILRGASNLTWRSDVLVEYQGEGRNVTDPTCPSLSPGVSQCFPDCVCEDAYNNTYACVRTMSALWNLQYCEFDDQEVFVEVYNLTADPDQITNIAKTIDPELLGKMNYRLMMLQSCSGPTCRTPGVFDPGYRFDPRLMFSNRGSVRTRRFSKHLL

Approximately 90 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 10% Glycerol, pH 8.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.