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  3. Glypican-1/GPC1 蛋白, Human (S486A, S488A, S490A, HEK293, His)

Glypican-1/GPC1 蛋白, Human (S486A, S488A, S490A, HEK293, His)

目录号: HY-P704638
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Glypican-1 (GPC1) 是一种含有硫酸乙酰肝素的细胞表面蛋白聚糖。Glypican-1/GPC1 蛋白, Human (S486A, S488A, S490A, HEK293, His) 是重组的 Glypican-1/GPC1 蛋白,由 HEK293 表达,带有 C-His 标签。

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描述

Glypican-1 (GPC1) is a heparan sulfate-containing cell surface proteoglycan. Glypican-1/GPC1 Protein, Human (S486A, S488A, S490A, HEK293, His) is the recombinant human-derived Glypican-1/GPC1 protein, expressed by HEK293, with C-His labeled tag.

研究背景

Glypican-1 (GPC1) 蛋白是一种细胞表面蛋白聚糖,其特征是与硫酸乙酰肝素相关。它通过硫酸乙酰肝素侧链结合 alpha-4 (V) 胶原蛋白,并在雪旺细胞髓鞘形成中发挥作用。此外,GPC1 可以作为朊病毒蛋白 PRPN(C) 转化为 PRNP(Sc) 的催化剂,通过与两种形式的 PRPN 结合,将它们靶向脂筏,并促进它们的相互作用。此外,GPC1 对于正确的骨骼肌分化至关重要,因为它将 FGF2 隔离在脂筏中,防止其与受体 (FGFR) 结合并抑制 FGF 介导的信号传导。

种属

Human

表达系统

HEK293

标签

C-His

蛋白编号

P35052-1 (D24-S530, S486A, S488A, S490A)

基因 ID

2817

蛋白结构
N-term
GPC1 (D24-S530, S486A, S488A, S490A)
Accession # P35052-1
His
C-term
同用名
rHuGlypican-1/GPC1, His; Glypican-1; GPC1
预测分子量
57.9 kDa.
分子量

Approximately 58-61 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

糖基化修饰
Yes
纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

Glypican-1/GPC1 Protein, Human (S486A, S488A, S490A, HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Glypican-1/GPC1 Protein, Human (S486A, S488A, S490A, HEK293, His)
目录号:
HY-P704638
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