Glypican-2/GPC2 蛋白, Human (HEK293, Fc)

Glypican-2/GPC2 is a cell surface proteoglycan that interacts with PTN to promote neurite outgrowth. Heparan sulfate of GPC2 binds PTN, releasing PTPRS from CSPG, thereby enabling PTPRS to bind GPC2. Glypican-2/GPC2 Protein, Human (HEK293, Fc) is the recombinant human-derived Glypican-2/GPC2 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of Glypican-2/GPC2 Protein, Human (HEK293, Fc) is 530 a.a., with molecular weight of ~35 kDa.

Glypican-2/GPC2 蛋白是一种含有硫酸乙酰肝素的细胞表面蛋白聚糖，与发育中神经元运动行为相关的功能有关。GPC2 通过其硫酸乙酰肝素与 PTN 相互作用，通过促进 PTN 与蛋白聚糖硫酸软骨素的结合来促进神经突生长。这反过来会释放硫酸软骨素蛋白聚糖 (CSPG) 的 PTPRS，使 PTPRS 与 GPC2 的硫酸乙酰肝素结合。此外，GPC2 的硫酸乙酰肝素链与 MDK 的相互作用先被肝素抑制，然后再被硫酸软骨素 E 抑制，从而诱导 GPC2 聚集。这种聚集在硫酸乙酰肝素链的促进下，诱导神经元细胞粘附和神经突生长。GPC2 复杂的相互作用突出了它在介导神经元发育过程中关键过程中的作用，并表明它通过复杂的分子相互作用参与调节细胞粘附和神经突生长。

Human

HEK293

C-hFc

Q8N158 (S24-S553)

221914  [NCBI]

Approximately 35 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized a 0.22 μm filtered solution of Tris with Glycine, Arginine and NaCl, pH 7.5. Normally trehalose is added as protectant before lyophilization.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.