GM-CSF 蛋白, Canine

The CSF2 protein is a cytokine that plays a critical role in the regulation of immune responses and hematopoiesis. It stimulates the production and differentiation of white blood cells, including macrophages and granulocytes. GM-CSF Protein, Canine is the recombinant canine-derived GM-CSF protein, expressed by E. coli , with tag free. The total length of GM-CSF Protein, Canine is 127 a.a., with molecular weight of ~15.11 kDa.

CSF2蛋白，也称为GM-CSF，在刺激不同谱系的造血前体细胞（例如粒细胞、巨噬细胞、嗜酸性粒细胞和红细胞）的生长和分化中发挥着至关重要的作用。它作为单体发挥作用，并与 GM-CSF 受体形成信号复合物。该受体复合物由两个α、两个β和两个配体亚基组成，排列成十二聚体结构，具有两个头对头的六聚体。

Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED₅₀ for this effect is 7.016 ng/ml, corresponding to a specific activity is 1.42×10^5 units/mg.

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  Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED₅₀ for this effect is 7.016 ng/mL, corresponding to a specific activity is 1.42×10⁵ units/mg.

Canine

E. coli

Tag Free

P48749-1 (A18-K144)

403923  [NCBI]

APTRSPTLVTRPSQHVDAIQEALSLLNNSNDVTAVMNKAVKVVSEVFDPEGPTCLETRLQLYKEGLQGSLTSLKNPLTMMANHYKQHCPPTPESPCATQNINFKSFKENLKDFLFNIPFDCWKPVKK

Approximately 15.11 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.