GM-CSF 蛋白, Mouse (HEK293, His)

The GM-CSF protein acts as a potent cytokine that coordinates the growth and differentiation of hematopoietic precursor cells of different lineages, including granulocytes, macrophages, eosinophils, and erythrocytes. Structurally, GM-CSF exists as a monomer and its signaling is mediated through a dodecamer complex. GM-CSF Protein, Mouse (HEK293, His) is the recombinant mouse-derived GM-CSF protein, expressed by HEK293 , with C-6*His labeled tag. The total length of GM-CSF Protein, Mouse (HEK293, His) is 124 a.a., with molecular weight of 18-30 kDa.

粒细胞巨噬细胞集落刺激因子 (GM-CSF) 是一种细胞因子，因其在刺激不同谱系的造血前体细胞（包括粒细胞、巨噬细胞、嗜酸性粒细胞和红细胞）的生长和分化方面的作用而闻名。在结构上，GM-CSF以单体形式存在。其信号传导机制涉及与 GM-CSF 受体复合物的相互作用，该复合物形成由两个头对头六聚体组成的十二聚体，涉及两个 α、两个 β 和两个配体亚基。通过这种复杂的受体复合物，GM-CSF 协调造血的调节，有助于多种血细胞类型的发育和成熟。

1. The cell proliferation assay using FDC-P1 cells has an ED₅₀ value of 40-170 pg/mL.
2. Measured in a cell proliferation assay using THP-1 human erythroleukemic cells. The ED₅₀ for this effect is 24.54 pg/mL, corresponding to a specific activity is 4.075×10⁷ units/mg.

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  Measured in a cell proliferation assay using THP‑1 human erythroleukemic cells.The ED₅₀ for this effect is 24.54 pg/mL, corresponding to a specific activity is 4.075×10⁷ units/mg.

Mouse

HEK293

C-6*His

P01587 (A18-K141)

12981  [NCBI]

APTRSPITVTRPWKHVEAIKEALNLLDDMPVTLNEEVEVVSNEFSFKKLTCVQTRLKIFEQGLRGNFTKLKGALNMTASYYQTYCPPTPETDCETQVTTYADFIDSLKTFLTDIPFECKKPGQK

18-30 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.