GMP HGF 蛋白, Human (HEK293, His)

HGF Protein is a multifunctional glycoprotein. By binding to the transmembrane tyrosine kinase receptor Met, HGF Protein plays a key role in cell proliferation, migration, differentiation and tissue repair. Abnormalities of HGF Protein are closely associated with tumor growth, metastasis and organ fibrosis. GMP HGF Protein, Human (HEK293, His) is a recombinant GMP-grade HGF protein expressed by HEK293 and tagged with C-6*His^[1][2][3].

HGF 是一种主要由间充质细胞分泌的多功能细胞因子。HGF 通过与跨膜酪氨酸激酶受体 Met 结合，激活下游信号通路。HGF 在胚胎发育中不可或缺，敲除相关基因会导致胚胎致死。HGF 具备诱导细胞增殖、迁移、形态发生和血管生成等多种生物学功能。HGF 参与肝、肾、肺等多器官的再生与修复，可通过抗凋亡、抗纤维化、抗炎等作用改善器官损伤。同时，HGF/Met 信号通路异常激活与肿瘤侵袭、转移密切相关。HGF 具有双重功能，既能促进组织修复，又能通过拮抗机制抑制肿瘤进展^[1][2][3]。

HGF 蛋白 (Human; 0-10 ng/mL; 72 h) 在原代大鼠和人的肝细胞中促进了 DNA 的合成^[4]。

HGF 蛋白 (Human; 500 μg; 动脉内注射; 2 次或 3 mg; 静脉注射; 5 次) 在兔后肢缺血模型中可诱导血管生成，显著促进了侧支血管生成和提高了血管造影评分^[5]。

Human

HEK293

C-6*His

P14210(Q32-S728)

3082

Full Length of Mature Protein

QRKRRNTIHEFKKSAKTTLIKIDPALKIKTKKVNTADQCANRCTRNKGLPFTCKAFVFDKARKQCLWFPFNSMSSGVKKEFGHEFDLYENKDYIRNCIIGKGRSYKGTVSITKSGIKCQPWSSMIPHEHSFLPSSYRGKDLQENYCRNPRGEEGGPWCFTSNPEVRYEVCDIPQCSEVECMTCNGESYRGLMDHTESGKICQRWDHQTPHRHKFLPERYPDKGFDDNYCRNPDGQPRPWCYTLDPHTRWEYCAIKTCADNTMNDTDVPLETTECIQGQGEGYRGTVNTIWNGIPCQRWDSQYPHEHDMTPENFKCKDLRENYCRNPDGSESPWCFTTDPNIRVGYCSQIPNCDMSHGQDCYRGNGKNYMGNLSQTRSGLTCSMWDKNMEDLHRHIFWEPDASKLNENYCRNPDDDAHGPWCYTGNPLIPWDYCPISRCEGDTTPTIVNLDHPVISCAKTKQLRVVNGIPTRTNIGWMVSLRYRNKHICGGSLIKESWVLTARQCFPSRDLKDYEAWLGIHDVHGRGDEKCKQVLNVSQLVYGPEGSDLVLMKLARPAVLDDFVSTIDLPNYGCTIPEKTSCSVYGWGYTGLINYDGLLRVAHLYIMGNEKCSQHHRGKVTLNESEICAGAEKIGSGPCEGDYGGPLVCEQHKMRMVLGVIVPGRGCAIPNRPGIFVRVAYYAKWIHKIILTYKVPQS

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of 20mM Tris-HCl, 500mM NaCl, pH 8.0.

<10 EU/mg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in injection water.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Cecchi F, et al. Targeting the HGF/Met signalling pathway in cancer. Eur J Cancer. 2010 May;46(7):1260-70.  [Content Brief]

  [2]. Stella MC, et al. HGF: a multifunctional growth factor controlling cell scattering. Int J Biochem Cell Biol. 1999 Dec;31(12):1357-62.  [Content Brief]

  [3]. Nakamura T, et al. The discovery of hepatocyte growth factor (HGF) and its significance for cell biology, life sciences and clinical medicine. Proc Jpn Acad Ser B Phys Biol Sci. 2010;86(6):588-610.  [Content Brief]

  [4]. Strain AJ, et al. Native and recombinant human hepatocyte growth factors are highly potent promoters of DNA synthesis in both human and rat hepatocytes. J Clin Invest. 1991 May;87(5):1853-7.  [Content Brief]

  [5]. Morishita R, et al. Therapeutic angiogenesis induced by human recombinant hepatocyte growth factor in rabbit hind limb ischemia model as cytokine supplement therapy. Hypertension. 1999 Jun;33(6):1379-84.  [Content Brief]