GMP IFN-gamma 蛋白, Human (HEK293)

IFN-gamma Protein is a cytokine belonging to the interferon family, secreted by activated immune cells such as T cells and NK cells. IFN-gamma Protein plays a key role in the immune system, exerting activities including regulating immune responses, antiviral effects, and antitumor effects. GMP IFN-gamma Protein, Human (HEK293) is a recombinant GMP-grade IFN-gamma protein expressed by HEK293, untagged but glycosylated^[1][2][3].

IFN-gamma 是一种具有免疫调节、抗肿瘤和抗病毒特性的细胞因子。IFN-gamma 可激活巨噬细胞和 NK 细胞、调节 T 细胞分化并参与炎症反应。IFN-gamma 能诱导抗病毒蛋白抑制病毒复制并增强感染细胞的免疫识别，还可通过抑制肿瘤细胞增殖、激活免疫细胞及抑制肿瘤血管生成发挥抗肿瘤作用。IFN-gamma 同时可促进 MHC 分子表达以提升免疫细胞对病原体和异常细胞的识别效率，此外还参与骨髓造血干细胞的分化与成熟调节^[1][2][3]。

IFN-gamma 蛋白 (Human; 100 ng/mL; 72 h) 在卵巢癌细胞系 PEO1 中引起细胞死亡和 G₂ 期细胞周期阻滞^[4]。
IFN-gamma 蛋白 (Human; 0.1-1 nM) 在人单核细胞培养中诱导多核巨细胞形成，并增强 H₂O₂ 生成和溶酶体酶活性^[5]。
IFN-gamma 蛋白 (Human; 1-100 ng/mL; 4-18 h) 在 LPS 刺激的人单核细胞中抑制 IL-10 mRNA 和蛋白表达，剂量依赖性上调 TNF-α 转录及蛋白水平^[6]。

The specific activity is >2×10⁷ IU/mg.

Human

HEK293

Tag Free

P01579 (Q24-Q166)

3458  [NCBI]

Full Length of Mature Protein

QDPYVKEAENLKKYFNAGHSDVADNGTLFLGILKNWKEESDRKIMQSQIVSFYFKLFKNFKDDQSIQKSVETIKEDMNVKFFNSNKKKRDDFEKLTNYSVTDLNVQRKAIHELIQVMAELSPAAKTGKRKRSQMLFRGRRASQ

16&18&25 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 4% Mannitol, 2% Sucrose, 0.02% Tween80, pH 7.4.

<0.01 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in injection water.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Kursunel MA, et al. The untold story of IFN-γ in cancer biology. Cytokine Growth Factor Rev. 2016 Oct;31:73-81.  [Content Brief]

  [2]. Chesler DA, et al. The role of IFN-gamma in immune responses to viral infections of the central nervous system. Cytokine Growth Factor Rev. 2002 Dec;13(6):441-54.  [Content Brief]

  [3]. Goedhart M, et al. Interferon-Gamma Impairs Maintenance and Alters Hematopoietic Support of Bone Marrow Mesenchymal Stromal Cells. Stem Cells Dev. 2018 May 1;27(9):579-589.  [Content Brief]

  [4]. Razaghi A, et al. Improved therapeutic efficacy of mammalian expressed-recombinant interferon gamma against ovarian cancer cells. Exp Cell Res. 2017 Oct 1;359(1):20-29.  [Content Brief]

  [5]. Weinberg JB, et al. Recombinant human gamma-interferon induces human monocyte polykaryon formation. Proc Natl Acad Sci U S A. 1984 Jul;81(14):4554-7.  [Content Brief]

  [6]. Donnelly RP, et al. Inhibition of IL-10 expression by IFN-gamma up-regulates transcription of TNF-alpha in human monocytes. J Immunol. 1995 Aug 1;155(3):1420-7.  [Content Brief]