GMP IL-4 蛋白, Human (HEK293)

GMP IL-4 Protein, Human (HEK293) is a recombinant GMP-grade protein produced by HEK293 cells with C-His tag. IL-4 is an anti-inflammatory cytokine acting as a pleiotropic regulator of numerous immune and inflammatory processes.

白细胞介素-4 (IL-4)，也称为 B 细胞刺激因子-1，是一种细胞因子，在调节炎症和免疫反应中发挥重要作用。它通常由辅助 T 2 淋巴细胞、肥大细胞、嗜酸性粒细胞和嗜碱性粒细胞分泌，在神经系统疾病（例如多发性硬化症、脊髓损伤）中发挥保护作用。IL-4 诱导幼稚辅助 T 细胞分化为 Th2 细胞。这种细胞因子与 IL-4 受体结合，而 IL-4 受体也与另一种细胞因子白细胞介素 13 (IL-13) 结合，这可能解释了 IL-4 和 IL-13 的重叠功能。将 IL-4 应用在小鼠受损神经上，可将 F4/80+ 巨噬细胞从促炎 M1 转变为抗炎 M2 表型，从而合成阿片肽（甲硫氨酸脑啡肽、β-内啡肽和强啡肽 A 1-17）^[1][2]。

The specific activity is >1×10⁷ U/mg.

Human

HEK293

Tag Free

P05112 (H25-S153)

3565  [NCBI]

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Approximately 18.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<0.1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in injection water.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Le HV, et al. Isolation and characterization of multiple variants of recombinant human interleukin 4 expressed in mammalian cells. J Biol Chem. 1988 Aug 5;263(22):10817-23.  [Content Brief]

  [2]. Melih Ö Celik, et al. IL-4 induces M2 macrophages to produce sustained analgesia via opioids. JCI Insight. 2020 Feb 27;5(4):e133093.  [Content Brief]