GMP SCF 蛋白, Human (HEK293, His)

SCF proteins are ligands for the KIT receptor-type protein tyrosine kinase and regulate a variety of cellular processes, including survival, proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration, and melanogenesis. Binding to KIT activates signaling pathways involving PIK3R1, AKT1, GRB2, RAS, RAF1, MAP kinase, STAT family members, and PLCG1, producing diacylglycerol and inositol 1,4,5-trisphosphate. GMP SCF Protein, Human (HEK293, His) is the recombinant human-derived SCF protein, expressed by HEK293 , with C-6*His labeled tag. The total length of GMP SCF Protein, Human (HEK293, His) is 189 a.a., with molecular weight of 30-50 kDa.

GMP 干细胞因子 (SCF) 蛋白作为受体型蛋白酪氨酸激酶 KIT 的配体，在多种细胞过程的调节中发挥着关键作用。其功能涵盖细胞存活和增殖、造血、干细胞维持、配子发生、肥大细胞发育、迁移和黑素生成的控制。与 KIT 结合后，GMP SCF 激活多种信号通路，包括 PIK3R1 的磷酸化和随后激酶 AKT1 的激活。这种相互作用还会触发涉及 GRB2、RAS、RAF1 和 MAP 激酶 MAPK1/ERK2 和/或 MAPK3/ERK1 的信号级联。此外，GMP SCF 和 KIT 促进 STAT 家族成员（STAT1、STAT3 和 STAT5）以及 PLCG1 的激活，从而产生细胞信号分子二酰甘油和肌醇 1,4,5-三磷酸。GMP SCF 与其他细胞因子（可能是白细胞介素）协同作用，与 KIT 形成非共价连接的同二聚体和异四聚体，促进 KIT 二聚化并随后通过自磷酸化激活。

Measured in a cell proliferation assay using TF-1 human erythroleukemic. The specific activity is > 5×10 ⁴ U/mg.

Human

HEK293

C-6*His

P21583 (E26-H214)

4254  [NCBI]

EGICRNRVTNNVKDVTKLVANLPKDYMITLKYVPGMDVLPSHCWISEMVVQLSDSLTDLLDKFSNISEGLSNYSIIDKLVNIVDDLVECVKENSSKDLKKSFKSPEPRLFTPEEFFRIFNRSIDAFKDFVVASETSDCVVSSTLSPEKDSRVSVTKPFMLPPVAASSLRNDSSSSNRKAKNPPGDSSLH

30-50 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<0.1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.