1. 重组蛋白
  2. Viral Proteins
  3. envelope glycoprotein gp120 蛋白, SIV (ADN41251, HEK293, His)

envelope glycoprotein gp120 蛋白, SIV (ADN41251, HEK293, His)

目录号: HY-P75159
产品使用指南

envelope glycoprotein gp120 蛋白是暴露在 HIV 包膜表面的糖蛋白。Gp120 对于病毒感染至关重要,因为它可以促进 HIV 进入宿主细胞。gp120 还可能通过影响 T 细胞对病毒的免疫反应来促进病毒持久性和持续的 HIV 感染。envelope glycoprotein gp120 蛋白, SIV (ADN41251, HEK293, His) 是重组的 包膜糖蛋白 gp120 蛋白,由 HEK293 表达,带有 C-His 标签。envelope glycoprotein gp120 蛋白, SIV (ADN41251, HEK293, His) 全长 509 个氨基酸,分子量约为 ~60 kDa。

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描述

envelope glycoprotein gp120 Protein is a glycoprotein exposed on the surface of the HIV envelope. Gp120 is essential for viral infection as it facilitates HIV entry into the host cell. gp120 may also be facilitating viral persistence and continuing HIV infection by influencing the T cell immune response to the virus. envelope glycoprotein gp120 Protein, SIV (ADN41251, HEK293, His) is the recombinant Virus-derived envelope glycoprotein gp120 protein, expressed by HEK293 , with C-His labeled tag. The total length of envelope glycoprotein gp120 Protein, SIV (ADN41251, HEK293, His) is 509 a.a., with molecular weight of ~60 kDa.

研究背景

包膜糖蛋白 gp120 (gp120) 是暴露在 HIV 包膜表面的糖蛋白。Gp120 对于病毒感染至关重要,因为它可以促进 HIV 进入宿主细胞,它在附着于特定细胞表面受体方面发挥着至关重要的作用。这些受体是 DC-SIGN、硫酸乙酰肝素蛋白聚糖以及与 CD4 受体的特异性相互作用,特别是在辅助 T 细胞上。gp120 还可能通过影响 T 细胞对病毒的免疫反应来促进病毒持久性和持续的 HIV 感染。在这个过程中可能涉及几种机制,gp120 与 T 细胞的 CD4 受体结合,促进病毒进入 CD4+ 细胞并消灭它们。Gp120 从病毒膜上脱落并在淋巴组织中大量积累。它可以诱导细胞凋亡,并通过抑制抗病毒 CTL 反应来严重改变对病毒的免疫反应,从而阻碍 HIV 的清除[1]

种属

Virus

表达系统

HEK293

标签

C-His

蛋白编号

ADN41251 (I20-R528)

基因 ID

/

蛋白结构
N-term
HIV-1 gp160 (I20-R528)
Accession # ADN41251
His
C-term
同用名
Simian immunodeficiency virus (SIV) (isolate 216.94.A2) gp120 Protein
分子量

Approximately 60 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

envelope glycoprotein gp120 Protein, SIV (ADN41251, HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
envelope glycoprotein gp120 Protein, SIV (ADN41251, HEK293, His)
目录号:
HY-P75159
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