GPR56 蛋白, Human (HEK293, His)

GPR56, a cell adhesion receptor, mediates cell-matrix adhesion in developing neurons and hematopoietic stem cells. It acts as a receptor for collagen III (COL3A1), influencing cortical development and hematopoietic stem cell maintenance. Additionally, GPR56 plays a pivotal role in cancer progression by inhibiting angiogenesis via PRKCA-mediated signaling and is essential for testis development. GPR56 Protein, Human (HEK293, His) is the recombinant human-derived GPR56 protein, expressed by HEK293 , with C-His labeled tag.

GPR56 蛋白作为细胞粘附中关键的受体发挥作用，并可能参与细胞间相互作用。它在神经元和造血干细胞发育过程中介导细胞基质粘附方面发挥着至关重要的作用。在发育中的大脑中，GPR56 作为胶原蛋白 III/COL3A1 的受体，有助于调节皮质发育，并在维持软脑膜基底膜完整性和皮质层压方面发挥特定作用。与 COL3A1 配体的相互作用抑制神经元迁移，并通过与 GNA13 和可能的 GNA12 偶联激活 RhoA 通路。此外，GPR56 还涉及骨髓生态位内造血干细胞和/或白血病干细胞的维持。值得注意的是，GPR56 在癌症进展中发挥着关键作用，通过 PRKCA 介导的信号通路抑制 VEGFA 产生和血管生成，以及激活 VEGFA 产生以促进血管生成，从而表现出双重功能。此外，GPR56 被证明在睾丸发育中至关重要，突显了其对各种细胞过程的多样化和重要贡献。

Measured by the ability of the immobilized protein to enhance the adhesion of T98G human glioblastoma cells to human Fibronectin. When 4 x 10^4 cells/well are added to plates coated with human Fibronectin (0.1 µg/mL) and rhGPR56 (10 µg/well, 100 µL/well) for 45 minutes at 37° C there is a 1.30 fold increase in adhesion to human fibronectin induced by the presence of rhGPR56.

Human

HEK293

C-His

Q9Y653-2/NP_958933.1 (R26-V342)

9289  [NCBI]

Partial

RGHREDFRFCSQRNQTHRSSLHYKPTPDLRISIENSEEALTVHAPFPAAHPASRSFPDPRGLYHFCLYWNRHAGRLHLLYGKRDFLLSDKASSLLCFQHQEESLAQGPPLLATSVTSWWSPQNISLPSAASFTFSFHSPPHTAAHNASVDMCELKRDLQLLSQFLKHPQKASRRPSAAPASQQLQSLESKLTSVRFMGDMVSFEEDRINATVWKLQPTAGLQDLHIHSRQEEEQSEIMEYSVLLPRTLFQRTKGRSGEAEKRLLLVDFSSQALFQDKNSSQVLGEKVLGIVVQNTKVANLTEPVVLTFQHQLQPKNV

45-70 kDa

• 
  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.