1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. GPT2 蛋白, Rat (sf9, His)

GPT2 蛋白, Rat (sf9, His)

目录号: HY-P75797
产品使用指南

GPT2 蛋白由 L-丙氨酸:2-酮戊二酸转氨酶活性预测,并参与胰岛素反应、糖异生和睾酮反应。它位于细胞外空间和线粒体中,是 2 型糖尿病的生物标志物,与人类 GPT2 直系同源。GPT2 蛋白, Rat (sf9, His) 是重组的 GPT2 蛋白,由 Sf9 insect cells 表达,带有 C-His 标签。GPT2 蛋白, Rat (sf9, His) 全长 522 个氨基酸,分子量约为 ~48 kDa。

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描述

GPT2 protein is predicted by L-alanine:2-oxoglutarate aminotransferase activity and is involved in insulin response, gluconeogenesis, and testosterone response. It is located in the extracellular space and mitochondria, is a biomarker for type 2 diabetes, and is orthologous to human GPT2. GPT2 Protein, Rat (sf9, His) is the recombinant rat-derived GPT2 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of GPT2 Protein, Rat (sf9, His) is 522 a.a., with molecular weight of ~48 kDa.

研究背景

GPT2 蛋白预计可实现 L-丙氨酸:2-酮戊二酸转氨酶活性并参与各种过程,包括细胞对胰岛素刺激的反应、糖异生的正向调节以及对睾酮的反应。它位于细胞外空间和线粒体中。GPT2 被认为是 2 型糖尿病的生物标志物,是人类 GPT2(谷氨酸-丙酮酸转氨酶 2)的直系同源对应物。值得注意的是,该基因表现出偏向表达,在肾上腺 (RPKM 312.5)、肝脏 (RPKM 220.8) 和其他八个组织中观察到显着水平,强调了其在不同组织的代谢过程和信号通路中的特定作用。

种属

Rat

表达系统

Sf9 insect cells

标签

C-His

蛋白编号

NP_001012057 (M1-S522)

基因 ID

307759  [NCBI]

蛋白结构
N-term
GPT2 (M1-S522)
Accession # NP_001012057
His
C-term
同用名
Alanine aminotransferase 2; ALT2; GPT 2; AAT2
分子量

Approximately 48 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

GPT2 Protein, Rat (sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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