2. 重组蛋白
  3. Enzymes & Regulators
  4. Oxidoreductases (EC 1)
  5. GRE3 蛋白, Saccharomyces cerevisiae

GRE3 蛋白, Saccharomyces cerevisiae

目录号: HY-P701871
产品使用指南

GRE3 蛋白是一种醛糖还原酶，可将细胞毒性化合物甲基乙二醛 (MG) 还原为丙酮醇和 (R)-乳醛，尤其是在应激条件下。MG 由磷酸二羟丙酮合成，参与细胞周期调节和应激适应。GRE3 蛋白, Saccharomyces cerevisiae 是重组的 GRE3 蛋白，由 E. coli 表达，不带标签。GRE3 蛋白, Saccharomyces cerevisiae 全长 327 个氨基酸。

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描述

The GRE3 protein is an aldose reductase that reduces the cytotoxic compound methylglyoxal (MG) to acetol and (R)-lactaldehyde, especially under stress conditions. MG is synthesized from dihydroxyacetone phosphate and is involved in cell cycle regulation and stress adaptation. GRE3 Protein, Saccharomyces cerevisiae is the recombinant GRE3 protein, expressed by E. coli , with tag free. The total length of GRE3 Protein, Saccharomyces cerevisiae is 327 a.a., .
研究背景

GRE3 蛋白作为醛糖还原酶发挥作用，具有广泛的底物特异性，能够将细胞毒性化合物甲基乙二醛 (MG) 还原为丙酮醇和 (R)-乳醛，特别是在应激条件下。甲基乙二醛是通过磷酸二羟丙酮糖酵解的旁路合成的，与细胞周期调节和应激适应有关。在戊糖发酵酵母中，GRE3 催化木糖还原为木糖醇。虽然纯化的酶可以进行该反应，但酿酒酵母无法以木糖作为唯一碳源茁壮成长，这表明其生理功能更可能面向甲基乙二醛还原。这种解毒细胞毒性化合物和参与代谢途径的双重作用强调了 GRE3 在细胞应激反应和适应中的多功能性和重要性。
种属

Others
表达系统

E. coli
标签

Tag Free
蛋白编号

P38715 (M1-A327)
基因 ID

856504
蛋白结构
N-term
GRE3 (M1-A327)
Accession # P38715
C-term
同用名
GRE3; NADPH-dependent aldose reductase GRE3; AR; Genes de respuesta a estres protein 3; NADPH-dependent aldo-keto reductase GRE3; Xylose reductase
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

GRE3 Protein, Saccharomyces cerevisiae 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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