1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Chemokine & Receptors
  4. CXC Chemokines
  5. GRO-alpha
  6. GRO-alpha/CXCL1 蛋白, Human (CHO)

GRO-alpha/CXCL1 蛋白, Human (CHO)

目录号: HY-P7363
COA 产品使用指南

CXCL1 (趋化因子 (C-X-C 基序) 配体 1),也称为 GRO-α、NAP-3 或MGSA,属于 CXC 趋化因子亚家族。CXCL1 参与许多炎症性疾病的发展,包括血管生成的诱导和嗜中性粒细胞的募集。CXCL1 由许多细胞类型产生,并激活 CXCR2CXCR1。GRO-alpha/CXCL1 蛋白, Human (CHO) 是在 CHO 细胞中产生的,由 73 个氨基酸 (A35-N107) 组成。

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描述

CXCL1 (Chemokine (C-X-C motif) ligand 1), also known as GRO alpha, NAP-3 or MGSA, belongs to the sub-family of CXC chemokine. CXCL1 is involved in the development of many inflammatory diseases, including the induction of angiogenesis and recruitment of neutrophils. CXCL1 is produced by many cell types, and activates CXCR2 and, at high levels, CXCR1[1]. GRO-alpha/CXCL1 Protein, Human (CHO) is produced in CHO cells, and consists of 73 amino acids (A35-N107).

研究背景

CXCL1,也称为 GRO-α,是一种最初从人类黑色素瘤细胞中分离出来的多肽。CXCL1 通过与其相应的 G 蛋白偶联受体 CXCR2 特异性结合,充当中性粒细胞的关键化学引诱剂。CXCL1 调节血管生成、肿瘤发生和伤口愈合。一般来说,CXCL1 水平在正常生理条件下极低,而在炎症条件下则大幅升高[2][3]
人类 CXCL1 的氨基酸序列小鼠和大鼠CXCL1蛋白同源性较低。
翻译后,合成的CXCL1前体长度为107个氨基酸。信号肽从其 N 末端被去除,从而将前体缩短为 73 个氨基酸。还可以从 C 末端去除另外两个氨基酸。此外,CXCL1 中的所有四个半胱氨酸残基形成两个二硫桥。二硫桥为 CXCL1 提供了适当的结构,这决定了该趋化因子的特性。分泌后,CXCL1 进行进一步的蛋白水解加工,从而调节该趋化因子的活性。从 N 末端去除 3 个、4 个或 5 个氨基酸,分别产生 CXCL1(4-73)、CXCL1(5-73) 和 CXCL1(6-73)。这使 CXCL1 活性增加了 30 倍,这是通过其诱导处理细胞趋化性的能力来衡量的。迄今为止,已经发现了三种CXCL1受体——CXCR1、CXCR2和非典型趋化因子受体1(ACKR1)。通过 NF-κB 激活,IL-1β、TNF-α 和 IL-17 等细胞因子可增加 CXCL1 的表达。CXCL1 可结合成生物活性二聚体,主要通过 CXCR2/IL-8 RB[1] 发出信号。
CXCL1 表达被致癌物诱导后,通过募集中性粒细胞参与炎症反应。这会导致慢性炎症。除了增加增殖之外,CXCL1 还诱导癌细胞迁移,特别是 EMT。CXCL1由淋巴管内皮细胞(LEC)产生,在淋巴管生成过程中使肿瘤细胞迁移到淋巴管,导致淋巴结转移。CXCL1 是中性粒细胞的趋化因子。此外,它还导致这些细胞从骨髓中动员起来。CXCL1 还可以诱导调节性 T 细胞 (Treg) 和 MSC 招募到肿瘤微环境中。CXCL1 另一个同样重要的特性是其诱导血管生成的能力[1]

体外研究
(In Vitro)

Recombinant human CXCL1 (0, 1, 5, and 10 ng/mL; 6-24 h) promotes IL-6 expression in a dose- and time-dependent manner, and triggers the transcriptional activities of c-Jun in human primary synovial fibroblasts[2].
Recombinant human CXCL1 (50 and 100 ng/mL; 16 h) significantly induces HUVEC proliferation in a concentration-dependent manner[3].

生物活性

Measured in a cell proliferation assay using HUVEC. The ED50 for this effect is 8.199 ng/mL, corresponding to a specific activity is 1.220×105 units/mg.

  • Measured in a cell proliferation assay using HUVEC. The ED50 for this effect is 8.199 ng/mL, corresponding to a specific activity is 1.220×105 units/mg.
种属

Human

表达系统

CHO

标签

Tag Free

蛋白编号

P09341 (A35-N107)

基因 ID
蛋白结构
N-term
CXCL1 (A35-N107)
Accession # P09341
C-term
中文名
重组人 CXC 趋化因子 1 (CHO)
同用名
rHuGRO/CXCL1; NAP-3; MGSA; C-X-C motif chemokine 1; SCYB1
氨基酸序列

ASVATELRCQCLQTLQGIHPKNIQSVNVKSPGPHCAQTEVIATLKNGRKACLNPASPIVKKIIEKMLNSDKSN

分子量

Approximately 7.8 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized after extensive dialysis against PBS.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

GRO-alpha/CXCL1 Protein, Human (CHO) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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