1. 重组蛋白
  2. Enzymes & Regulators
  3. Oxidoreductases (EC 1)
  4. gucD 蛋白, Bacillus subtilis (His, Strep)

gucD 蛋白, Bacillus subtilis (His, Strep)

目录号: HY-P701906
产品使用指南

gucD蛋白是一种重要的酶,可催化L-犬尿氨酸不可逆脱氨生成犬尿酸(KA)。该过程是色氨酸分解代谢途径的一部分,并产生 KA,以其对兴奋性氨基酸受体的广谱拮抗活性而闻名。gucD 蛋白, Bacillus subtilis (His, Strep) 是重组的 枯草芽孢杆菌 gucD 蛋白,由 E. coli 表达,带有 N-Strep, N-6*His 标签。gucD 蛋白, Bacillus subtilis (His, Strep) 全长 487 个氨基酸。

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描述

The gucD protein is an important enzyme that catalyzes the irreversible ammonia removal of L-kynurenine to generate kynurenic acid (KA). This process is part of the tryptophan catabolic pathway and produces KA, known for its broad-spectrum antagonistic activity at excitatory amino acid receptors. gucD Protein, Bacillus subtilis (His, Strep) is the recombinant gucD protein, expressed by E. coli , with N-Strep, N-6*His labeled tag. The total length of gucD Protein, Bacillus subtilis (His, Strep) is 487 a.a., .

研究背景

KYAT1 蛋白协调 L-色氨酸代谢物 L-犬尿氨酸的不可逆转氨作用,最终形成犬尿酸 (KA)。该过程在色氨酸分解代谢途径中具有重要意义,并产生 KA,因其对三种离子型兴奋性氨基酸受体等的广谱拮抗活性而闻名。除了在色氨酸代谢中的作用之外,KYAT1 还参与半胱氨酸缀合物从特定卤代烯烃和烷烃向反应性代谢物的转化。此外,KYAT1 催化 L-(硒代)半胱氨酸的 S-缀合物和 Se-缀合物的 β-消除,导致 CS 或 C-Se 键的断裂。KYAT1 的多种酶活性强调了其参与关键代谢途径和外源性加工。

种属

Others

表达系统

E. coli

标签

N-Strep;N-6*His

蛋白编号

P42236 (S2-P488)

基因 ID

938406

蛋白结构
N-term
Strep-6*His
gucD (S2-P488)
Accession # P42236
C-term
同用名
gucD; Alpha-ketoglutaric semialdehyde dehydrogenase; alphaKGSA dehydrogenase; 2; 5-dioxovalerate dehydrogenase
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

gucD Protein, Bacillus subtilis (His, Strep) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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gucD Protein, Bacillus subtilis (His, Strep)
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