1. 重组蛋白
  2. Enzymes & Regulators Biotinylated Proteins
  3. Lyases (EC 4)
  4. GUCY2C/Guanylyl cyclase C 蛋白, Human (Biotinylated, HEK293, His-Avi)

GUCY2C/Guanylyl cyclase C 蛋白, Human (Biotinylated, HEK293, His-Avi)

目录号: HY-P77944
COA 产品使用指南

GUCY2C 是一种鸟苷酸环化酶,催化 GTP 合成 cGMP,并作为大肠杆菌热稳定肠毒素的受体,刺激表达哺乳动物细胞中 cGMP 的积累。此外,内源性肽鸟苷蛋白和尿鸟苷蛋白可激活 GUCY2C。研究证实,这种多方面的作用强调了细胞信号传导中复杂的调节机制以及 GUCY2C 对各种刺激的反应。GUCY2C/Guanylyl cyclase C 蛋白, Human (Biotinylated, HEK293, His-Avi) 是重组的 GUCY2C/鸟苷酸环化酶 C 蛋白,由 HEK293 表达,带有 C-Avi, C-His 标签。GUCY2C/Guanylyl cyclase C 蛋白, Human (Biotinylated, HEK293, His-Avi) 全长 407 个氨基酸,分子量为 55-65 kDa。

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规格 价格 是否有货 数量
20 μg ¥2350 In-stock
50 μg ¥5100 In-stock
100 μg ¥8680 询价
> 100 μg   询价  

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描述

GUCY2C, a guanylyl cyclase, catalyzes cGMP synthesis from GTP and acts as a receptor for E. coli heat-stable enterotoxin, stimulating cGMP accumulation in expressing mammalian cells. Additionally, endogenous peptides guanylin and uroguanylin activate GUCY2C. This multifaceted role underscores intricate regulatory mechanisms in cellular signaling and GUCY2C's responsiveness to various stimuli, as confirmed by studies. GUCY2C/Guanylyl cyclase C Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived GUCY2C/Guanylyl cyclase C protein, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of GUCY2C/Guanylyl cyclase C Protein, Human (Biotinylated, HEK293, His-Avi) is 407 a.a., with molecular weight of 55-65 kDa.

研究背景

研究证明,GUCY2C 是一种鸟苷酸环化酶,可作为 GTP 合成环 (cGMP) 的催化剂。此外,该蛋白还充当大肠杆菌热稳定肠毒素的受体,其中肠毒素显着刺激表达 GUCY2C 的 cin 哺乳动物细胞的积累。此外,GUCY2C 被内源性肽、鸟苷蛋白和尿鸟苷蛋白激活。这种多方面的作用强调了细胞信号传导中涉及 GUCY2C 的复杂调节机制及其对各种刺激的反应。

生物活性

Immobilized Anti-GUCY2C Antibody at 1 μg/mL (100μL/Well) on the plate. Dose response curve for Biotinylated Human GUCY2C with the EC50 of 24.1-33.6 ng/mL determined by ELISA.

种属

Human

表达系统

HEK293

标签

C-Avi;C-His

蛋白编号

P25092 (S24-Q430)

基因 ID
蛋白结构
N-term
GUCY2C (S24-Q430)
Accession # P25092
His-Avi
C-term
同用名
Guanylyl cyclase C; GC-C; STAR; GUCY2C; GUC2C; STA receptor; DIAR6; EC 4.6.1; GCC; GUC2CEC 4.6.1.2; MUCIL
分子量

55-65 kDa

纯度

Greater than 95% as determined by Tris-Bis PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

GUCY2C/Guanylyl cyclase C Protein, Human (Biotinylated, HEK293, His-Avi) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
GUCY2C/Guanylyl cyclase C Protein, Human (Biotinylated, HEK293, His-Avi)
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