HA/Hemagglutinin 蛋白, H1N1 (EPI2397519, sf9, His)

HA/Hemagglutinin Protein, H1N1 (EPI2397519, sf9, His) is the recombinant virus-derived HA/Hemagglutinin protein, expressed by Sf9 insect cells, with C-10*His tag.

HA/血凝素蛋白通过与细胞表面上含唾液酸的受体结合，启动病毒颗粒与宿主细胞的附着，从而在病毒进入中发挥关键作用。这种附着导致病毒颗粒内化，大约三分之二的病毒颗粒经历网格蛋白依赖性内吞作用，大约三分之一利用网格蛋白和小窝蛋白独立途径。除了在病毒进入中的作用之外，HA 作为 I 类病毒融合蛋白，在确定宿主范围限制和毒力方面也发挥着至关重要的作用。它的职责延伸到介导内吞病毒颗粒膜与内体膜的融合，从而允许渗透到细胞质中。HA2 亚基响应内体中的低 pH 值而发生不可逆的构象变化，从而促进融合疏水肽的释放。值得注意的是，有能力的融合孔的形成需要几个三聚体的协作。

1.Measured by its ability to agglutinate guinea pig red blood cells.
2.CR9114 captured on Protein A chip, can bind Influenza A H1N1 (A/Victoria/4897/2022) Hemagglutinin / HA Protein, His Tag with an affinity constant of 0.434 nM as determined in an SPR assay (Biacore 8K) (Routinely tested).

Virus

Sf9 insect cells

C-10*His

EPI2397519 (M1-I530)

/

Approximately 65.49 kDa, based on SDS-PAGE under reducing conditions.

Greater than 95% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of 20mM Tris, 300mM NaCl, 10% glycerol, pH 8.0.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.